Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood
Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood
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DOI：
10.1016/j.bmc.2004.08.004
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发表时间：
2004-11-01
影响因子：
3.5
通讯作者：
Chen, ST
中科院分区：
文献类型：
--
作者：
Chien, CA;Cheng, JL;Chen, ST
在我们之前的研究中，从灵芝的水溶性提取物中分离出的含焦糖蛋白部分（F3）被证明可以刺激小鼠脾细胞增殖和细胞因子的表达。我们现在进一步研究F3对单核细胞（MNCs）免疫表型表达的影响。人脐带血（hUCB） MNCs经F3 （10 ~ 100 mug/mL）处理7 d后，CD14(+)CD26（+）单核/巨噬细胞、CD83(+)CD1a（+）树突状细胞和CD16(+)CD56(+) nk细胞的数量分别是未处理对照组的2.9倍、2.3倍和1.5倍（p < 0.05）。b细胞群无明显变化。然而，在较高浓度的F3（100马克杯/毫升）存在下，T细胞的生长受到轻微抑制，CD3标记物的表达下降了20%。我们还发现，F3对体外人细胞没有危害，F3处理后nk细胞介导的细胞毒性在效应/靶细胞比（E/T） 20:1时显著增强31.7% (p < 0.01)，但在E/T 5:1时没有改变。(C) 2004 Elsevier Ltd.版权所有。
In our previous study, a fucose-containing glycoprotein fraction (F3), isolated from the water-soluble extracts of Ganoderma lucidum, was shown to stimulate mice spleen cell proliferation and cytokine expression. We now further investigate the effect of F3 on the immunophenotypic expression in mononuclear cells (MNCs). When human umbilical cord blood (hUCB) MNCs were treated with F3 (10-100 mug/mL) for 7 days, the population of CD14(+)CD26(+) monocyte/macrophage, CD83(+)CD1a(+) dendritic cells, and CD16(+)CD56(+) NK-cells were 2.9, 2.3, and 1.5 times higher than those of the untreated controls (p < 0.05). B-cell population has no significant change. T cell growth was, however, slightly inhibited and CD3 marker expression decreased similar to20%, in the presence of higher concentrations of F3 (100 mug/mL). We also found that F3 is not harmful to human cells in vitro, and after F3 treatment, NK-cell-mediated cytotoxicity was significantly enhanced by 31.7%, (p < 0.01) at effector/target cell ratio (E/T) 20:1, but was not altered at E/T 5:1. (C) 2004 Elsevier Ltd. All rights reserved.