Genes involved in invasion and metastasis of gastric cancer identified by array-based hybridization and serial analysis of gene expression
Genes involved in invasion and metastasis of gastric cancer identified by array-based hybridization and serial analysis of gene expression
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DOI：
10.1159/000086627
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发表时间：
2005-01-01
期刊：
影响因子：
3.5
通讯作者：
Yasui, W
中科院分区：
文献类型：
--
作者：
Oue, N;Aung, PP;Yasui, W
胃癌（GC）仍然是一个严重的健康问题，并且仍然是全球第二大常见的致命癌症类型。全面的基因表达分析可能有助于识别与胃癌侵袭/转移相关的基因。其中，基于芯片的杂交和基因表达系列分析（SAGE）是目前最常见的方法。在过去的 3 年里，利用基于阵列的杂交和 SAGE 进行了几项大规模基因表达研究，并鉴定了一些基因。这篇综述描述了与 GC 侵袭/转移相关的基因，这些基因已通过基于阵列的杂交和 SAGE 鉴定出来。我们将基于阵列的杂交鉴定的基因的表达水平与我们的 SAGE 数据进行了比较。此外，通过40个GC样品的定量RT-PCR进一步研究了SAGE获得的候选基因的表达。 MIA 和 GW112 在 40 个 GC 样本中的 10 个（25%）和 22 个（55%）中过表达，这两个基因的过表达分别与肿瘤分期相关。我们还讨论了HMB/两性蛋白在GC侵袭和转移中的意义。版权所有 (C) 2005 S. Karger AG，巴塞尔。
Gastric cancer (GC) is still a serious health problem and remains the second most common type of fatal cancer worldwide. Comprehensive gene expression analyses may be useful to identify genes associated with invasion/metastasis in GC. Among them, array-based hybridization and serial analysis of gene expression (SAGE) are currently the most common approaches. Over the past 3 years, several large-scale gene expression studies with array-based hybridization and SAGE have been performed and several genes have been identified. This review describes genes associated with invasion/metastasis in GC which have been identified by array-based hybridization and SAGE. We compared the expression levels of the genes identified by array-based hybridization with our SAGE data. In addition, expression of the candidate genes obtained by SAGE was further investigated by quantitative RT-PCR of 40 GC samples. MIA and GW112 were overexpressed in 10 (25%) and 22 (55%) of 40 GC samples, and the overexpression of these two genes was associated with tumor stage, respectively. We also discuss the significance of HMB/amphoterin in invasion and metastasis of GC. Copyright (C) 2005 S. Karger AG, Basel.