Boron Affects Immune Function Through Modulation of Splenic T Lymphocyte Subsets, Cytokine Secretion, and Lymphocyte Proliferation and Apoptosis in Rats
Boron Affects Immune Function Through Modulation of Splenic T Lymphocyte Subsets, Cytokine Secretion, and Lymphocyte Proliferation and Apoptosis in Rats
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硼通过调节大鼠脾 T 淋巴细胞亚群、细胞因子分泌以及淋巴细胞增殖和凋亡来影响免疫功能
DOI：
10.1007/s12011-017-0932-3
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发表时间：
2017-08-01
影响因子：
3.9
通讯作者：
Li, Kui
中科院分区：
文献类型：
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作者：
Jin, Erhui;Li, Shenghe;Li, Kui
本研究揭示了大鼠模型中硼的作用机制，为硼的合理使用提供了科学依据。这些发现是通过研究饮用水中硼（10、20、40、80、160、320和640 mg/L或1.5、3、6、12、24、48和96 mg/kg体重）对大鼠血清免疫球蛋白（igg）、脾细胞因子、淋巴细胞亚群以及淋巴细胞增殖和凋亡的影响而得出的。在饮水中添加20(3)和40 (6)mg/L （mg/kg BW）的硼可显著提高大鼠血清IgG浓度、脾脏IFN-γ和IL-4表达以及脾脏CD3+、CD4+和增殖细胞核抗原(PCNA)+细胞的数量。饮水中添加40 mg/L （6 mg/kg BW）的硼也显著提高了脾脏IL-2表达和CD4+/CD8+细胞比值，减少了脾脏CD8+细胞数量。添加80 mg/L （12 mg/kg BW）硼显著提高了脾脏中CD3+和PCNA+细胞数量（P< 0.05），降低了IL-10表达。添加320（48）和640 (96)mg/L （mg/kg BW）硼显著降低血清IgG浓度；脾IL-2、IL-10表达；CD3+、CD4+、PCNA+细胞数量；增加脾脏CD8+和caspase-3+细胞数量，促进CD3+细胞中caspase-3表达。综上所述，在大鼠饮水中添加20(3)和40(6)mg/L （mg/kg BW）的硼可以显著增强大鼠的体液和细胞免疫功能，而浓度超过320 mg/L （48 mg/kg BW）的硼则会对免疫功能产生抑制甚至毒性作用。上述结果显示了低剂量和高剂量硼对免疫功能的u型反应特征，提示在人和动物食品中适当添加硼可作为免疫调节剂。
This study demonstrated the mechanisms of boron effects in a rat model and provided a scientific basis for the rational of boron use. These findings were achieved by investigating the effects of boron (10, 20, 40, 80, 160, 320, and 640 mg/L in drinking water or 1.5, 3, 6, 12, 24, 48, and 96 mg/kg BW) on rat serum immunoglobulins (IgGs), splenic cytokines, lymphocyte subsets, as well as on lymphocyte proliferation and apoptosis. Addition of 20 (3) and 40 (6) mg/L (mg/kg BW) of boron to drinking water significantly increased rat serum IgG concentrations, splenic IFN-γ and IL-4 expression as well as the number of splenic CD3+, CD4+and proliferating cell nuclear antigen (PCNA)+cells. Supplementation of drinking water with 40 mg/L (6 mg/kg BW) boron also markedly increased splenic IL-2 expression and the CD4+/CD8+cell ratio and reduced splenic CD8+cell number. Supplementation with 80 mg/L (12 mg/kg BW) boron significantly increased CD3+and PCNA+cell numbers (P< 0.05) and decreased the IL-10 expression in the spleen. Addition of 320 (48) and 640 (96) mg/L (mg/kg BW) boron markedly reduced the serum IgG concentrations; splenic IL-2 and IL-10 expression; the number of CD3+, CD4+and PCNA+cells; and increased the number of splenic CD8+and caspase-3+cells and promoted caspase-3 expression in CD3+cells. In conclusion, these findings suggest that the supplementation of rat drinking water with 20(3) and 40(6) mg/L (mg/kg BW) boron can markedly enhance humoral and cellular immune functions, while boron concentrations above 320 mg/L (48 mg/kg BW) can have an inhibitory effect or even toxicity on immune functions. These results exhibit a U-shaped response characteristic of low and high doses of boron supplementation on immune function and imply that proper boron supplementation in food for humans and animals could be used as an immunity regulator.