Strigolactone perception and deactivation by a hydrolase receptor DWARF14
Strigolactone perception and deactivation by a hydrolase receptor DWARF14
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DOI：
10.1038/s41467-018-08124-7
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发表时间：
2019-01-14
影响因子：
16.6
通讯作者：
Yamaguchi, Shinjiro
中科院分区：
文献类型：
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作者：
Seto, Yoshiya;Yasui, Rei;Yamaguchi, Shinjiro
由于植物激素受体DWARF14(D14)是一种能裂解植物激素的α/β水解酶，因此对植物激素的感知机制一直备受争议。在这里，我们通过对拟南芥D14的SL结合和水解的时间过程分析表明，未切割的SL的水平与激活信号状态的诱导强烈相关。此外，我们还表明，缺乏酶活性的atD14(D218A)催化突变体仍然能够以SL依赖的方式补充atd14突变体的表型。我们的结论是，完整的SL分子触发了D14的活性信号状态，我们还描述了D14通过信号传递后的水解性降解来失活生物活性的SLS。综上所述，这些结果表明，D14是一个双功能受体，负责生物活性SLS的感知和失活。
The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an alpha/beta-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14(D218A) catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.