Quantification of bleomycin-induced murine lung damage in vivo with micro-computed tomography
Quantification of bleomycin-induced murine lung damage in vivo with micro-computed tomography
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DOI：
10.1016/j.acra.2006.08.011
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发表时间：
2006-12-01
影响因子：
4.8
通讯作者：
Cody, Dianna D.
中科院分区：
文献类型：
--
作者：
Cavanaugh, Dawn;Travis, Elizabeth L.;Cody, Dianna D.
基本原理和目标。我们探索了无创体内锥形束微计算机断层扫描 (micro-CT)，以可视化和量化小鼠整个肺体积的纤维化和炎症损伤。材料和方法。我们使用博来霉素在体内诱导肺损伤，并将显微 CT 的结果与组织学测量结果进行比较。 10 只 C5713L/6 小鼠气管内给予 5 U/kg 博来霉素。在给予博莱霉素之前和处死之前，对七只幸存的小鼠进行了微型 CT 扫描。分析所得图像以进行肺体积测量。最终扫描后，对所有肺部进行组织学检查，并对肺部损伤进行量化。每只小鼠的受损肺组织区域在显微 CT 图像和组织学切片之间进行匹配。结果。根据显微CT和组织学计算的肺损伤百分比具有相关性（r(2) = 0.49，r = 0.64，P = 0.12），并且它们各自的分布均值没有差异（P > 0.05）。结论。这项研究表明，微型 CT 是预测博来霉素引起的肺损伤的一种有前途的替代方法。胸部 CT 图像体积允许进行整体组织采样，这在气管内施用博来霉素可能发生不均匀肺损伤时可能很有用。
Rationale and Objectives. We explored noninvasive, in vivo cone-beam microcomputed tomography (micro-CT) to visualize and quantify fibrotic and inflammatory damage over the entire lung volume of mice.Materials and Methods. We used bleomycin to induce pulmonary damage in vivo and compared the results from micro-CT with histologic measurements. Ten C5713L/6 mice were given 5 U/kg bleomycin intratracheally. Seven surviving mice were scanned with micro-CT before administration of bleomycin, and again before sacrifice. The resulting images were analyzed for lung volume measurements. After the final scan, all lungs were examined histologically and pulmonary damage was quantified. Damaged lung tissue regions were matched between micro-CT images and histologic sections for each mouse.Results. The percent lung damage calculated from micro-CT and histology were correlated (r(2) = 0.49, r = 0.64 with P = 0.12), and the means of their respective distributions were not different (P > 0.05).Conclusion. This study shows that micro-CT is a promising alternative to predicting lung damage caused by bleomycin. CT image volumes of the thorax allow for global tissue sampling, which may be useful when following nonuniform lung damage that can occur from intratracheal administration of bleomycin.