Anti-inflammatory effects of fucoidan through inhibition of NF-κB, MAPK and Akt activation in lipopolysaccharide-induced BV2 microglia cells
Anti-inflammatory effects of fucoidan through inhibition of NF-κB, MAPK and Akt activation in lipopolysaccharide-induced BV2 microglia cells
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DOI：
10.1016/j.fct.2011.04.020
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Choi, Yung Hyun
中科院分区：
文献类型：
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作者：
Park, Hye Young;Han, Min Ho;Choi, Yung Hyun
褐藻糖胶是从褐藻中提取的一种硫酸化多糖，具有多种体内生物活性，但其抗炎作用的细胞和分子机制尚不清楚。在本研究中，我们研究了岩藻糖胶对内毒素诱导的BV2小胶质细胞产生促炎介质的抑制作用。结果表明，岩藻糖胶可显著抑制脂多糖刺激的BV2小胶质细胞产生过量的一氧化氮(NO)和前列腺素E-2(PGE(2))。还可抑制诱导型一氧化氮合酶(INOS)、环氧合酶(COX)-2、单核细胞趋化蛋白-1(MCP-1)和促炎细胞因子，包括白介素1β(IL-1β)和肿瘤坏死因子(TNF)-α的表达。此外，岩藻糖胶还通过抑制核因子-kappaB(NF-kappa B)的激活和下调细胞外信号调节激酶(ERK)、c-jun氨基末端激酶(JNK)、p38丝裂原活化蛋白激酶(MAPK)和AKT通路而显示出抗炎作用。这些发现表明，岩藻糖胶可能为治疗伴有小胶质细胞激活的神经退行性疾病提供实质性的治疗潜力。(C)2011爱思唯尔有限公司。保留所有权利。
Fucoidan, a sulfated polysaccharide extracted from brown seaweed, displays a wide variety of internal biological activities; however, the cellular and molecular mechanisms underlying fucoidan's anti-inflammatory activity remain poorly understood. In this study, we investigated the inhibitory effects of fucoidan on production of lipopolysaccharide (LPS)-induced pro-inflammatory mediators in BV2 microglia. Our data indicated that fucoidan treatment significantly inhibited excessive production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) in LPS-stimulated BV2 microglia. It also attenuated expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, monocyte chemoattractant protein-1 (MCP-1), and pro-inflammatory cytokines, including interleukin-1 beta (IL-1 beta) and tumor necrosis factor (TNF)-alpha. Moreover, fucoidan exhibited anti-inflammatory properties by suppression of nuclear factor-kappa B (NF-kappa B) activation and down-regulation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and AKT pathways. These finding suggest that fucoidan may offer substantial therapeutic potential for treatment of neurodegenerative diseases that are accompanied by microglial activation. (C) 2011 Elsevier Ltd. All rights reserved.