The growth inhibitory effect of conjugated linoleic acid on MCF-7 cells is related to estrogen response system
The growth inhibitory effect of conjugated linoleic acid on MCF-7 cells is related to estrogen response system
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DOI：
10.1016/s0304-3835(97)00192-4
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发表时间：
1997-06-24
期刊：
影响因子：
9.7
通讯作者：
Fernandes, G
中科院分区：
文献类型：
--
作者：
Durgam, VR;Fernandes, G
共轭亚油酸（CLA）已被证明对培养的MCF-7人乳腺癌细胞有直接的抑癌作用。然而，所涉及的机制尚未完全阐明。在这项研究中，我们研究了抑制剂的作用是否与MCF-7细胞的雌激素反应性有关。我们的研究结果表明，与ER阴性的MDA-MB-231细胞相比，CLA选择性地抑制ER阳性MCF-7细胞的增殖。细胞周期研究表明，与对照组和亚油酸（LA）处理的MCF-7细胞相比，CLA处理的MCF-7细胞保持在G(0)/G(1)期的比例更高。CLA还能抑制MCF-7细胞中c-myc的表达。这些结果表明，CLA可能通过干扰激素调节的有丝分裂途径来抑制MCF-7细胞的生长。我们首次报道了饮食因子CLA在体外ER阳性乳腺癌细胞增殖中参与激素介导的有丝分裂途径的调节。爱尔兰爱思唯尔科学有限公司出版。
Conjugated linoleic acid (CLA) has been shown to have a direct oncostatic action on MCF-7 human breast cancer cells in culture. However, the mechanism involved is not fully elucidated. In this study we have examined whether the inhibitor action is related to the estrogen responsiveness of MCF-7 cells. Our results demonstrate that CLA selectively inhibits proliferation of ER positive MCF-7 cells as compared with ER negative MDA-MB-231 cells. Cell cycle studies indicated that a higher percentage of CLA treated MCF-7 cells remained in the G(0)/G(1) phase as compared to control and those treated with linoleic acid (LA). CLA also inhibited expression of c-myc in MCF-7 cells. These results demonstrate that CLA may inhibit MCF-7 cell growth by interfering with the hormone regulated mitogenic pathway. We are: reporting for the first time the involvement of CLA, a dietary factor, in the regulation of hormone mediated mitogenic pathways in ER positive breast cancer cell proliferation in vitro. Published by Elsevier Science Ireland Ltd.