Areas 3a, 3b, and 1 of human primary somatosensory cortex 1. Microstructural organization and interindividual variability
Areas 3a, 3b, and 1 of human primary somatosensory cortex 1. Microstructural organization and interindividual variability
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DOI：
10.1006/nimg.1999.0440
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发表时间：
1999-07-01
期刊：
影响因子：
5.7
通讯作者：
Zilles, K
中科院分区：
文献类型：
--
作者：
Geyer, S;Schleicher, A;Zilles, K
本研究通过客观描绘细胞结构边界和随后的细胞结构分类来定义人类初级体感皮层的细胞结构区3a，3b和1。这避免了对微观结构差异的主观评价，而这是迄今为止在结构上定义皮质区域的唯一方法。将10个脑固定在福尔马林或Bodian固定剂中，包埋在石蜡中，在20 μ m处在冠状平面上整体切片，并进行细胞染色。通过自适应阈值法将细胞体从背景中分割出来。等距密度分布（125 μ m宽，间距300或150 μ m）提取垂直于软膜表面的皮质层II-VI和多元统计程序处理。相邻组的配置文件之间的形状的显着差异的位置与cytoarchitectonic模式。统计上显著的边界可以在一系列附近部分的相应位置处再现。它们与细胞染色切片中细胞结构的可见变化相匹配。3a区位于中央沟的底部，3b区位于中央后回的喙侧。1区位于冠部，向下延伸到中央后沟。然而，区域间边界与中央后回的宏观结构标志不匹配，并且它们相对于这些标志的位置在不同的大脑中有很大差异。因此，只有真正的显微结构分析才能确定这些皮层区域之间的边界。在区域3b和1内可以发现与细胞结构的可见变化不相关的其他显著边界。它们可能代表不同躯体感觉受体的躯体和/或皮质表征。(C)北京：科学出版社.
This study defines cytoarchitectonic areas 3a, 3b, and 1 of the human primary somatosensory cortex by objective delineation of cytoarchitectonic borders and ensuing cytoarchitectonic classification. This avoids subjective evaluation of microstructural differences which has so far been the only way to structurally define cortical areas. Ten brains were fixed in formalin or Bodian's fixative, embedded in paraffin, sectioned as a whole in the coronal plane at 20 mu m, and cell stained. Cell bodies were segmented from the background by adaptive thresholding. Equidistant density profiles (125 mu m wide, spacing 300 or 150 mu m) were extracted perpendicularly to the pial surface across cortical layers II-VI and processed with multivariate statistical procedures. Positions of significant differences in shape between adjacent groups of profiles were correlated with the cytoarchitectonic pattern. Statistically significant borders can be reproduced at corresponding positions across a series of nearby sections. They match visible changes in cytoarchitecture in the cell-stained sections. Area 3a lies in the fundus of the central sulcus, and area 3b in the rostral bank of the postcentral gyrus. Area 1 lies on its crown and reaches down into the postcentral sulcus. Interareal borders, however, do not match macrostructural landmarks of the postcentral gyrus, and they considerably vary in their positions relative to these landmarks across different brains. Hence, only genuine microstructural analysis can define the borders between these cortical areas. Additional significant borders which do not correlate with visible changes in cytoarchitecture can be found within areas 3b and 1. They may represent somatotopy and/or cortical representations of different somatosensory receptors. (C) 1999 Academic Press.