Immunohistochemical analysis of cytokeratin 7 expression in resting and proliferating biliary structures of rat liver
Immunohistochemical analysis of cytokeratin 7 expression in resting and proliferating biliary structures of rat liver
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DOI：
10.1002/hep.20858
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发表时间：
2005-10-01
期刊：
影响因子：
13.5
通讯作者：
Nagy, P
中科院分区：
文献类型：
--
作者：
Paku, S;Dezsö, K;Nagy, P
细胞角蛋白是最大的中间丝蛋白亚家族，包括 20 多种不同的基因产物，这些产物以上皮组织特异性方式表达。我们通过免疫组织化学和共聚焦显微镜研究了成年大鼠肝脏胆道系统中细胞角蛋白亚型的分布。在胆管树的较小分支（包括赫林管）中观察到细胞角蛋白 (CK) 19+/7- 胆管细胞群。给予 2-乙酰氨基芴 (AAF) 后，它们增殖，尽管后来典型的卵圆细胞表达 CK7。这一观察结果表明，具有这种细胞角蛋白表型的胆管细胞可能含有成体肝干细胞。 CK19+/7-胆管细胞在出生时并不存在于大鼠肝脏中，而是在出生后发育。在人类肝脏中没有观察到类似的细胞群。总之，我们提出CK19+/7-表型可能是大鼠肝脏中成体肝干细胞的特征，并且这些细胞是在出生后从头产生的。
Cytokeratins are the largest subfamily of intermediate filament proteins and include more than 20 different gene products, which are expressed in an epithelial tissue-specific manner. We studied by immunohistochemistry and confocal microscopy the distribution of cytokeratin subtypes in the biliary system of adult rat liver. A cytokeratin (CK) 19+/7- cholangiocyte population was observed in the smaller branches of the biliary tree including the canals of Hering. They proliferated after 2-acetaminofluorene (AAF) administration, although later the typical oval cells expressed CK7. This observation suggests that cholangiocytes with this cytokeratin phenotype may harbor adult hepatic stem cells. The CK19+/7- cholangiocytes were not present in the rat liver at birth, but developed postnatally. Similar cell populations were not observed in human livers. In conclusion, we propose that the CK19+/7- phenotype may be characteristic for adult hepatic stem cells in rat liver and that these cells are generated de novo after birth.