Eluting solution composition affects DNA double-strand break analysis by filter elution.
Eluting solution composition affects DNA double-strand break analysis by filter elution.
复制标题
洗脱液成分影响过滤器洗脱的 DNA 双链断裂分析。
DOI：
10.1080/09553008814552181
复制
发表时间：
1988
影响因子：
2.6
通讯作者：
Kazmar,ER
中科院分区：
文献类型：
--
作者：
Koval,TM;Kazmar,ER
采用过滤洗脱技术检测137Cs对中国仓鼠V79细胞DNA双链断裂的影响。在pH为7·2和9·6时，用两种不同的洗脱和洗脱溶液进行洗脱。这些数据与其他人在pH为7·2和9·6时的洗脱曲线不同的数据一致。此外，数据还表明，溶液组成对洗脱有显著的影响。
The filter elution technique was used to assay for137Cs-induced DNA double-strand breaks (dsb) in V79 Chinese hamster cells. The elutions were performed using two different sets of lysing and eluting solutions at pH 7·2 and 9·6. The data agree with those of others who have demonstrated differences in elution profiles at pH 7·2 and 9·6. In addition, the data indicate that solution composition has a significant effect on elution.