ULTRASTRUCTURE OF INVITRO OOCYTE MATURATION IN CATTLE
ULTRASTRUCTURE OF INVITRO OOCYTE MATURATION IN CATTLE
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DOI：
10.1530/jrf.0.0780615
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发表时间：
1986-11-01
期刊：
影响因子：
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通讯作者：
GREVE, T
中科院分区：
文献类型：
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作者：
HYTTEL, P;XU, KP;GREVE, T
在屠宰场通过抽吸小（1-6 mm）窦状卵泡从奶牛中收集卵丘-卵母细胞复合体。在不同的培养时间后，对复合物进行电子显微镜检查。卵丘扩张发生在12-18小时的文化和随之而来的卵丘细胞在卵周空间的预测扩大被视为。48 h时，最内层的卵丘细胞变平，与透明质膜紧密贴附。在卵母细胞中发生了以下变化：在0-3小时的培养物的卵周空间开发;在3-12小时的卵丘细胞突起和卵膜之间的连接断开，并观察到伴随的细胞核的破裂;在12-18小时的线粒体从周边位置移动到更均匀的空间分布和高尔基复合体的大小减小;在apprx。18 h，滑面内质网形成大的聚集体，周围有线粒体; 18-21 h，第一极体脱落; 24-40 h，皮质颗粒散开; 30-40 h，部分标本极体退化; 40-48 h，卵周间隙缩小; 48 h，有1个卵母细胞处于碎裂过程中。结论：卵母细胞的核质成熟在体外被刺激。然而，与体内成熟相比，注意到某些偏差。
Cumulus-oocyte complexes were collected from cows at an abattoir by aspiration from small (1-6 mm) antral follicles. After different periods of culture the complexes were processed for electron microscopy. Cumulus expansion occurred at 12-18 h of culture and concomitantly enlargement of cumulus cell projections in the perivitelline space was seen. At 48 h the innermost cumulus cells flattened and adhered tightly to the zona pellucida. In the oocyte the following changes occurred: at 0-3 h of culture the perivitelline space developed; at 3-12 h disconnection of the junctions between cumulus cell projections and the oolemma, and the concomitant breakdown of the nucleus was observed; at 12-18 h the mitochondria moved from a peripheral location to a more even spatial distribution and the Golgi complexes decreased in size; at .apprx. 18 h the smooth endoplasmic reticulum formed large aggregates surrounded by mitochondria; at 18-21 h the first polar body was abstricted; at 24-40 h the cortical granules spread; at 30-40 h the polar body degenerated in some specimens; at 40-48 h the perivitelline space decreased in size; and at 48 h one oocyte was in the process of fragmentation. It is concluded that nuclear and cytoplasmic oocyte maturation is stimulated in vitro. However, certain deviations were noticed compared to in-vivo maturation.