Nonlinear dielectric spectroscopy for label-free detection of respiratory activity in whole cells.
Nonlinear dielectric spectroscopy for label-free detection of respiratory activity in whole cells.
复制标题
非线性介电谱用于全细胞呼吸活动的无标记检测。
DOI：
10.1016/j.bios.2010.02.013
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发表时间：
2010
影响因子：
12.6
通讯作者：
MillerJr,JohnH
中科院分区：
文献类型：
--
作者：
Mercier,GTSkip;Palanisami,Akilan;MillerJr,JohnH
我们报告了一种新的电磁生物传感技术，用于检测悬浮在水溶液中的整个细胞的呼吸活动。在两个外部电极之间施加纯正弦电压以1至几kHz范围内的频率向水性细胞悬浮液施加振荡电场。使用动态信号分析仪测量两个内电极上的基波和高阶谐波响应。S.具有活性和非活性线粒体电子传递（呼吸）链的酿酒酵母（芽殖酵母）用于该研究。我们发现，测得的第三谐波的某些频率范围内显示出显着的时间变化，积极呼吸酵母，而很少有显着的变化，观察到在酵母与抑制呼吸活性，即突变酵母菌株或酵母在呼吸抑制剂的存在下。该方法具有进一步发展的潜力，以检测体外活组织中的呼吸活动，并可能在体内用于临床应用。
We report on a novel electromagnetic biosensing technique for detecting respiratory activity in whole cells suspended in aqueous solution. Application of a pure sinusoidal voltage between two outer electrodes applies an oscillatory electric field to the aqueous cell suspension at frequencies in the range of one to several kHz. The fundamental and higher order harmonic responses are measured across two inner electrodes using a dynamic signal analyzer. Aqueous suspensions of S. cerevisiae (budding yeast), with both active and inactive mitochondrial electron transport (respiratory) chains are employed for this study. We find that the measured third harmonic for certain frequency ranges shows significant temporal changes in actively respiring yeast, while little significant changes are observed in yeast with suppressed respiratory activity, i.e. mutant yeast strains or yeast in the presence of respiratory inhibitors. The method holds potential for further development to detect respiratory activity in live tissue in vitro and perhaps in vivo for clinical applications.