Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro
Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro
复制标题
高三尖杉酯碱、羟氯喹或shRNA及其组合三种治疗方法体外对抗冠状病毒PEDV
DOI：
10.1186/s12985-020-01342-w
复制
发表时间：
2020-06-03
期刊：
影响因子：
4.8
通讯作者：
Wang, Xiao-Jia
中科院分区：
文献类型：
--
作者：
Li, Cui-Cui;Wang, Xiao-Jia
背景冠状病毒科猪流行性腹泻病毒（PEDV）给养猪业造成了巨大的经济损失。目前还没有专门用于治疗冠状病毒或PEDV的药物。方法本研究以冠状病毒PEDV为模型，研究抗病毒药物。简要地说，我们研究了融合抑制剂tHR2、重组慢病毒递送的靶向保守M和N序列的shrna、同杉酸酯碱（HHT）和羟氯喹（HCQ）的抗病毒作用。结果50 μ M的HCQ和150 nM的HHT分别使TCID50的病毒滴度降低30倍和3.5倍，联合处理使TCID50的病毒滴度降低200倍。结论HHT和HCQ联合使用比HHT和HCQ单独使用具有更高的抗病毒活性。这些信息可能有助于开发有效控制PEDV感染的抗病毒策略。
Background Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There's currently no specific drug available for treatment of coronaviruses or PEDV. Method In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects. Results Treatment with HCQ at 50 mu M and HHT at 150 nM reduced virus titer in TCID50 by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID50 by 200 fold. Conclusion Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection.