Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis
Involvement of a Magnaporthe grisea serine/threonine kinase gene, MgATG1, in appressorium turgor and pathogenesis
复制标题
稻瘟病菌丝氨酸/苏氨酸激酶基因 MgATG1 参与附着胞膨压和发病机制
DOI：
10.1128/ec.00011-07
复制
发表时间：
2007-06-01
期刊：
影响因子：
--
通讯作者：
Lin, Fu-Cheng
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Hong;Lu, Jian-Ping;Lin, Fu-Cheng
我们从稻瘟病菌中分离到一个编码丝氨酸/苏氨酸蛋白激酶的MgATG 1基因。在三角洲Mgatg 1突变体，其中MgATG 1基因已被删除，自噬被阻断，突变体也表现出较少的脂滴在其分生孢子，较低的膨压的附着胞，和这样的缺陷在形态建成作为延迟启动和萌发较慢的分生孢子。由于附着胞的膨压较低，Delta Mgatg 1突变体失去了穿透和感染两种宿主植物，即水稻和大麦的能力。然而，恢复正常值的参数和感染能力，重新引入一个完整的拷贝的MgATG 1基因突变体。因此，自噬是分生孢子和附着胞形成过程中有机物质周转以及M.稻瘟病菌
We isolated an MgATG1 gene encoding a serine/threonine protein kinase from the rice blast fungus Magnaporthe grisea. In the Delta Mgatg1 mutant, in which the MgATG1 gene had been deleted, autophagy was blocked; the mutant also showed fewer lipid droplets in its conidia, lower turgor pressure of the appressorium, and such defects in morphogenesis as delayed initiation and slower germination of conidia. As a result of lower turgor pressure of the appressorium, the Delta Mgatg1 mutant lost its ability to penetrate and infect the two host plants, namely, rice and barley. However, normal values of the parameters and infective abilities were restored on reintroducing an intact copy of the MgATG1 gene into the mutant. Autophagy is thus necessary for turnover of organic matter during the formation of conidia and appressoria and for normal development and pathogenicity in M. grisea.