HOMOLOGOUS AND HETEROLOGOUS REGULATION OF 1,25-DIHYDROXYVITAMIN D-3 RECEPTOR MESSENGER-RNA LEVELS IN HUMAN OSTEOSARCOMA CELLS
HOMOLOGOUS AND HETEROLOGOUS REGULATION OF 1,25-DIHYDROXYVITAMIN D-3 RECEPTOR MESSENGER-RNA LEVELS IN HUMAN OSTEOSARCOMA CELLS
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DOI：
10.1016/0167-4781(91)90159-j
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发表时间：
1991-01-17
期刊：
影响因子：
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通讯作者：
MAENPAA, PH
中科院分区：
文献类型：
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作者：
MAHONEN, A;PIRSKANEN, A;MAENPAA, PH
激素受体的异源调节在激素受体文献中有详细描述。 我们感兴趣的是确定人类 1,25-二羟基维生素 D-3 受体 (hVDR) 和糖皮质激素受体 (GR)（类固醇/甲状腺激素受体家族的成员）是否受到其他类固醇和相关激素的异源调节。 我们使用人骨肉瘤细胞 (MG-63) 并测量雄激素、雌激素、糖皮质激素、黄体酮、甲状腺激素、维生素 A 和维生素 D 治疗后的 hVDR 和 GR mRNA 水平。 除雄激素和黄体酮外，每种激素都能够像人骨肉瘤细胞中的天然配体一样增加 hVDR mRNA 水平。 另一方面，GR基因表达不受这些激素的影响。 为了研究细胞是否响应 1,25(OH)2D3 处理而改变分化和增殖，我们还研究了 c-myc 和 c-fos 基因表达。 两个基因仅受 1,25(OH)2D3 调节。 1,25(OH)2D3在添加激素后4-12小时内略微增加c-fos mRNA的积累，而c-myc mRNA的增加出现在24小时。
The heterologous regulation of hormone receptors is well described in the hormone receptor literature. We were interested in determining whether human 1,25-dihydroxyvitamin D-3 receptor (hVDR) and glucocorticoid receptor (GR), members of the steroid / thyroid hormone receptor family, are heterologously regulated by other steroids and related hormones. We used human osteosarcoma cells (MG-63) and measured hVDR and GR mRNA levels after androgen, estrogen, glucorticoid, progesterone, thyroid hormone, vitamin A and vitamin D treatments. Each hormone, except androgen and progesterone, was capable of increasing hVDR mRNA levels like the natural ligand in human osteosarcoma cells. On the other hand, GR gene expression was not affected by these hormones. To study whether the cells responded to the 1,25(OH)2D3-treatment with changes in differentiation and proliferation, we also studied c-myc and c-fos gene expression. Both genes were only regulated by 1,25(OH)2D3. 1,25(OH)2D3 slightly increased the accumulation of c-fos mRNA within 4-12 h from the hormone addition, while the increase in c-myc mRNA appeared at 24 h.