Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture.
Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture.
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肿瘤促进剂抑制培养物中小鼠红白血病细胞的自发分化和诱导分化。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
P. Marks
中科院分区：
文献类型：
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作者：
H. Yamasaki;E. Fibach;U. Nudel;I. Weinstein;R. Rifkind;P. Marks
添加有效的肿瘤促进剂，12-O-十四烷酰基-佛波醇-13-乙酸酯（TPA），小鼠红白血病细胞系在悬浮培养抑制自发分化和分化诱导的双乙酰胺（HMBA），二甲基亚砜，或丁酸。抑制作用与细胞毒性无关，并且是可逆的。当测试几种植物二萜时，促肿瘤活性和抑制分化之间存在正相关。只有在分化之前添加TPA才能抑制HMBA诱导的分化，这通过将细胞从HMBA转移到不含HMBA的新鲜培养基后的分化评分来测定。TPA介导的分化抑制与珠蛋白mRNA积累的减少有关。
Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.