Effect of cell size on growth rates of two Skeletonema species and their competitive interactions
Effect of cell size on growth rates of two Skeletonema species and their competitive interactions
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细胞大小对两种骨条藻生长速率的影响及其竞争相互作用
DOI：
10.1111/jpy.12782
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发表时间：
2018
影响因子：
2.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Ando;Y. Katano;T.
我们研究了两种硅藻，日本双丝藻和杜氏双丝藻的生长速率和细胞大小减小-恢复过程的变化。即使在相同的条件下，生长率也在每天1.0到2.1之间波动。随着瓣膜直径的减小，速率从每天1.1增加到2.1;然而，当细胞大小达到启动孢子缺陷形成的阈值时，速率迅速下降到每天1.0。我们还使用两个物种的不同大小组合进行了共培养实验。使用两种物种的相同细胞密度（75个细胞· mL-1）开始实验，当分批培养达到指数晚期时，将培养物重新接种到新培养基中两次。当大的（瓣膜直径为17 μm）日本血吸虫细胞和小的（6 μm）多赫尔尼血吸虫细胞共培养时，在第16天，多赫尔尼血吸虫占总细胞密度的99%（多赫尔尼血吸虫：263，900个细胞· mL-1;日本血吸虫：2，000个细胞· mL-1）。相反，当小的（9 μm）日本血吸虫细胞和大的（15 μm）多氏血吸虫细胞共培养时，仅在13 d后，小的日本血吸虫细胞就占总细胞密度的97%（多氏血吸虫：1，900个细胞· mL−1;日本血吸虫：62，500个细胞· mL−1）。这项研究表明，硅藻的生长速率与细胞大小的协变，这种现象可能会决定物种之间的竞争结果。
We investigated shifts in growth rates and cell size reduction–restoration processes in two species of diatoms,Skeletonema japonicumandSkeletonema dohrnii. Growth rates greatly fluctuated from 1.0 to 2.1 per day, even under the same conditions. Rates increased from 1.1 to 2.1 per day with decreasing valve diameter; however, rates quickly decreased to 1.0 per day when cell size reached the threshold for initiating auxosporulation. We also conducted co‐culture experiments using different size combinations of the two species. The experiment was started using the same cell densities (75 cells · mL−1) of both species, and when batch cultures reached late exponential phase, the cultures were reinoculated twice into new medium. When large (valve diameter of 17 μm)S. japonicumcells and small (6 μm)S. dohrniicells were co‐cultured, theS. dohrniicontributed 99% of the total cell density on day 16 (S. dohrnii: 263,900 cells · mL−1;S. japonicum: 2,000 cells · mL−1). In contrast, when small (9 μm)S. japonicumcells and large (15 μm)S. dohrniicells were co‐cultured, smallS. japonicumcells accounted for 97% of the total cell density after only 13 d (S. dohrnii: 1,900 cells · mL−1;S. japonicum: 62,500 cells · mL−1). This study demonstrated that diatom growth rates covary with cell size, and this phenomenon potentially determines the outcome of competition between species.