Gas-liquid chromatographic determination of bupivacaine and lidocaine in plasma.
Gas-liquid chromatographic determination of bupivacaine and lidocaine in plasma.
复制标题
气相色谱法测定血浆中布比卡因和利多卡因。
作者：
E. Zylber‐katz;L. Granit;M. Levy
描述了一种改进的气液色谱法，用于测定布比卡因和利多卡因的血浆浓度，以赛克利嗪作为内标。提取过程不需要溶剂蒸发，从而克服了药物挥发的问题。可以测定低至 0.1 毫克/升的浓度。将血浆样品碱化并萃取到正己烷中，再萃取到少量的酸水相中，最后碱化后萃取到50微升二氯甲烷中。最终提取物通过气相色谱法在 5% OV-17 柱上进行分析。本方法的提取方案消除了内源性血浆成分的干扰。
A modified gas-liquid chromatographic method for determining plasma concentrations of bupivacaine and lidocaine is described, with cyclizine as an internal standard. The extraction procedure requires no solvent evaporation, thus overcoming the problem of drug volatility. Concentrations as low as 0.1 mg/liter can be determined. The plasma sample is made alkaline and extracted into n-hexane, re-extracted into a small volume of an aqueous acid phase, and finally extracted into 50 microliter of methylene chloride after alkalinization. The final extract is assayed by gas chromatography on a 5% OV-17 column. The extraction scheme of the present method eliminates interferences by endogenous plasma constituents.