Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodhius prolixus
Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodhius prolixus
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DOI：
10.1016/j.febslet.2005.09.035
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发表时间：
2005-11-07
期刊：
影响因子：
3.5
通讯作者：
Ferreira, ST
中科院分区：
文献类型：
--
作者：
Oliveira, MF;Kycia, SW;Ferreira, ST
血红色素（Hz）是一种血红素晶体，由吸血生物消化血红蛋白（Hb）产生，是血红素处置的主要机制。Hz的结构由血红素二聚体组成，通过相互铁-羧酸盐相互作用结合并通过氢键稳定。我们最近在血吸虫曼氏血吸虫（Schistosoma mansoni）和吻虫（Rhodnius prolixus）中描述了血红素晶体。在这里，我们表征了来自这两种生物体的血红素晶体的结构和形态，并将其与合成的P-血红素（β H）进行了比较。同步辐射X射线粉末衍射表明，所有血红素晶体具有相同的晶胞和结构。从S. mansoni和R. prolixus由组装在多晶球形结构中的非常规则的单元组成，与β H相比，显示出明显不同的表面形态。在这两种生物体中，Hz形成发生在脂滴样颗粒内部或与磷脂膜密切相关。这些结果表明，第一次，从这两个吸血生物体获得的天然Hz样品的结构和形态特征。此外，Hz的形成发生在密切相关的疏水环境似乎是这些生物体的共同趋势，可能是至关重要的，以产生非常规则的形状相，允许在肠道中的S的多晶组件的形成。mansoni和R.冗长(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Hemozoin (Hz) is a heme crystal produced upon the digestion of hemoglobin (Hb) by blood-feeding organisms as a main mechanism of heme disposal. The structure of Hz consists of heme dimers bound by reciprocal iron-carboxylate interactions and stabilized by hydrogen bonds. We have recently described heme crystals in the blood fluke, Schistosoma mansoni, and in the kissing bug, Rhodnius prolixus. Here, we characterized the structures and morphologies of the heme crystals from those two organisms and compared them to synthetic P-hematin (beta H). Synchrotron radiation X-ray powder diffraction showed that all heme crystals share the same unit cell and structure. The heme crystals isolated from S. mansoni and R. prolixus consisted of very regular units assembled in multicrystalline spherical structures exhibiting remarkably distinct surface morphologies compared to beta H. In both organisms, Hz formation occurs inside lipid droplet-like particles or in close association to phospholipid membranes. These results show, for the first time, the structural and morphological characterization of natural Hz samples obtained from these two blood-feeding organisms. Moreover, Hz formation occurring in close association to a hydrophobic environment seems to be a common trend for these organisms and may be crucial to produce very regular shaped phases, allowing the formation of multicrystalline assemblies in the guts of S. mansoni and R. prolixus. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.