Influence of laboratory-related and endoscopy-related factors on the assessment of serum pepsinogens and gastrin-17
Influence of laboratory-related and endoscopy-related factors on the assessment of serum pepsinogens and gastrin-17
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DOI：
10.1097/meg.0000000000000952
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发表时间：
2017-12-01
影响因子：
2.1
通讯作者：
Malfertheiner, Peter
中科院分区：
文献类型：
--
作者：
Goni, Elisabetta;Venerito, Marino;Malfertheiner, Peter
背景与目的血清胃蛋白酶原I（PGI）和胃蛋白酶原II（PGII）是检测萎缩性胃炎的无创参数。血清胃泌素-17（G-17）的诊断附加值仍不确定。本研究的目的是评估这些血清参数随时间的稳定性，并评估临床因素的影响，如上消化道（GI）内窥镜检查和肠道清洁，对血清PGI，PGII，和G-17 assessment.Patients和方法进行了前瞻性研究，在健康的个人和患者。对于稳定性分析，在添加和不添加稳定剂的情况下，在不同时间点处理了23份个体的血浆和血清样本。10例患者接受上消化道内镜检查，18例患者接受结肠镜检查前肠道清洁。结果血清和血浆中PGI、PGII和G-17水平差异无统计学意义。PGI和PGII血清水平随时间推移保持稳定。G-17与时间依赖性降解相关（P = 0.0001）。G-17稳定剂的加入显示稳定性没有改善。上消化道内镜检查和结肠镜检查前肠道准备与PGI和PGII的最小变化，而G-17显示患者特异性alteration.Conclusion PGI和PGII血清水平是稳定的，随着时间的推移。然而，G-17稳定性强烈依赖于处理和储存时间;因此，用于G-17分析的样品需要在采血后6小时内处理。上消化道内窥镜检查和结肠镜检查准备导致基础PGI、PGII和G-17水平的最小非显著变化。版权所有（C）2017威科医疗集团All rights reserved.
Background and aim Serum pepsinogen I (PGI) and pepsinogen II (PGII) are noninvasive parameters in the detection of atrophic gastritis. The diagnostic add-on value of serum gastrin-17 (G-17) remains uncertain. The aim of this study was to assess the stability of these serum parameters over time and to evaluate the influence of clinical factors, such as upper gastrointestinal (GI) endoscopy and bowel cleansing, on serum PGI, PGII, and G-17 assessment.Patients and methods A prospective study was carried out in healthy individuals and patients. For the stability analyses, the plasma and serum samples from 23 individuals were processed at different time points with and without the addition of a stabilizer. Ten patients were included to evaluate the influence of upper GI endoscopy and 18 patients to evaluate the effect of bowel cleansing before colonoscopy.Results PGI, PGII, and G-17 levels were not statistically different in the serum and plasma. PGI and PGII serum levels were stable over time. G-17 is associated with time-dependent degradation (P = 0.0001). The addition of the G-17 stabilizer showed no improvement in stability. Upper GI endoscopy and bowel preparation before colonoscopy were associated with minimal variations in PGI and PGII, whereas G-17 showed patient-specific alterations.Conclusion PGI and PGII serum levels are stable over time. However, G-17 stability is strongly dependent on the time of processing and storage; therefore, samples for G-17 analysis need to be processed no later than 6 h after blood collection. Upper GI endoscopy and colonoscopy preparation lead to minimal nonsignificant changes in basal PGI, PGII, and G-17 levels. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.