p53 protein expression in non-Hodgkin's lymphomas is infrequently related to p53 gene mutations
p53 protein expression in non-Hodgkin's lymphomas is infrequently related to p53 gene mutations
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DOI：
10.1111/j.1440-1827.1998.tb03822.x
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发表时间：
1998-01-01
影响因子：
2.2
通讯作者：
Akagi, T
中科院分区：
文献类型：
--
作者：
Oka, T;Sarker, AB;Akagi, T
在几种类型的淋巴瘤中发现了p53蛋白的积累。然而，p53基因突变在某些特定类型的淋巴瘤中很少被证实。在本研究中，p53免疫反应性和p53基因突变之间的相关性在一个大面板的非霍奇金淋巴瘤（NHL）的情况下，试图。应用免疫组化方法检测202例NHL中p53蛋白的表达。所有p53蛋白免疫组化阳性的病例均采用聚合酶链反应（PCR）单链构象多态性（SSCP）方法进行分析，以确定p53基因内的突变。为了确认突变，进行PCR扩增的p53基因片段的测序。202例NHL中有59例p53蛋白过表达，但SSCP分析仅4例p53蛋白表达发生改变，其中3例测序发现p53蛋白点突变。p53免疫反应性在高级别淋巴瘤中普遍较高。本研究的结果表明，免疫组化反应p53蛋白不是一个可靠的指标存在的p53基因外显子4-9的结构改变在NHL。
Accumulation of the p53 protein has been found in several types of lymphomas. However, p53 gene mutations have been infrequently demonstrated in some specific types of lymphomas. In the present study, a correlation between p53 immunoreactivity and p53 gene mutations in a large panel of non-Hodgkin's lymphoma (NHL) cases is attempted. A panel of 202 cases of NHL was evaluated by immunohistochemical staining for p53 protein. All cases that were immunohistochemically positive for p53 protein were analyzed by the polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) method to identify mutations within the p53 gene. In order to confirm the mutation, sequencing of PCR-amplified p53 gene segments was performed. Overexpression of p53 protein was found in 59 of the 202 cases of NHL, but only four of these 59 cases showed a shift on SSCP analysis, and point mutations were detected in three of them by the subsequent sequencing. p53 immunoreactivity was generally greater in high-grade lymphoma. The results of this study suggest that immunohistochemical reactivity for p53 protein is not a reliable indicator of the presence of their structural alterations of p53 gene exons 4-9 in NHL.