Association of Pneumococcal Carriage and Expression of Foxp3+Regulatory T Cells and Th17 Cells in the Adenoids of Children
Association of Pneumococcal Carriage and Expression of Foxp3+Regulatory T Cells and Th17 Cells in the Adenoids of Children
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DOI：
10.1159/000381724
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发表时间：
2015-01-01
期刊：
影响因子：
3.7
通讯作者：
Fu, Zhou
中科院分区：
文献类型：
--
作者：
Jiang, Xiao-li;Zhang, Guang-li;Fu, Zhou
背景：肺炎球菌在鼻咽部的携带是肺炎球菌疾病的主要传播途径和必要的先决条件。目的：我们分析了Foxp 3+调节性T（T-reg）细胞和Th 17细胞的表达与肺炎球菌阳性或阴性儿童腺样体中肺炎球菌携带之间的关系。方法：我们收集腺样体组织和鼻咽拭子样本，从儿童接受腺样体切除术。分离、培养腺样单核细胞，然后用从D39细菌菌株获得的培养物浓缩上清液（CCS）刺激。结果如下：在从肺炎球菌阳性组获得的腺样单核细胞群体中，Foxp 3 + T-reg细胞上调，Th 17细胞下调。CCS刺激后，肺炎球菌阳性组Foxp 3 + T-reg细胞的增量显著大于肺炎球菌阴性组，而Th 17细胞的增量小于肺炎球菌阴性组。这些结果与腺样单核细胞中Foxp 3 mRNA和视黄酸受体相关孤儿受体-γ t mRNA水平的变化一致。与肺炎球菌阳性组相比，肺炎球菌阴性组中腺样体组织中IL-17 A和IL-6的水平较高，而肺炎球菌阴性组中腺样体组织中TGF-β的水平较低。儿童肺炎球菌携带与腺样体Foxp 3 + T-reg和Th 17细胞的表达密切相关。结论：Foxp 3 + T-reg细胞的上调可能下调了腺样体中Th 17细胞的产生，导致肺炎链球菌的清除减少和慢性肺炎球菌携带。(C)2015 S. Karger AG，巴塞尔
Background: Pneumococcal carriage in the nasopharynx is a primary means of transmission and a necessary prerequisite for pneumococcal disease. Objectives: We analyzed the relationship between expressions of Foxp3+ regulatory T (T-reg) cells and Th17 cells, and pneumococcal carriage in the adenoids of children who were either positive or negative for pneumococci. Methods: We collected adenoidal tissue and nasopharyngeal swab samples from children undergoing an adenoidectomy. Adenoidal mononuclear cells were isolated, cultured and then stimulated with culture concentrated supernatant (CCS) obtained from a D39 bacterial strain. Results: Foxp3+ T-reg cells were upregulated and Th17 cells were downregulated in populations of adenoidal mononuclear cells obtained from the pneumococcus-positive group. Following CCS stimulation, the increment in Foxp3+ T-reg cells in the pneumococcus-positive group was significantly greater than that in the pneumococcus-negative group, while the increment in Th17 cells was less as compared to that in the pneumococcus-negative group. These results were consistent with variations in levels of Foxp3 mRNA and retinoic acid receptor-related orphan receptor-gamma t mRNA in adenoidal mononuclear cells. Levels of IL-17A and IL-6 in adenoid tissue were higher in the pneumococcus-negative group, and the levels of TGF-beta in adenoid tissue were lower in the pneumococcus-negative group compared to the pneumococcus-positive group. Pneumococcal carriage in children was closely associated with the expressions of Foxp3+ T-reg and Th17 cells in the adenoid. Conclusion: Upregulation of Foxp3+ T-reg cells might downregulate the production of Th17 cells in the adenoid, resulting in decreased scavenging of Streptococcus pneumoniae and chronic pneumococcal carriage. (C) 2015 S. Karger AG, Basel