Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support -: clinical study
Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support -: clinical study
复制标题
DOI：
10.1046/j.1365-4362.2003.01792.x
复制
发表时间：
2003-03-01
影响因子：
3.6
通讯作者：
Smetana, K
中科院分区：
文献类型：
--
作者：
Dvoránková, B;Holíková, Z;Smetana, K
背景广泛的伤口覆盖仍然是当代医学的一个挑战。我们证明了移植培养的角质形成细胞直接在一个聚合物培养支持在严重烧伤患者和营养性溃疡患者的皮肤缺损的治疗的临床试验的结果。重建表皮的形态和表型模式，包括基底层，以及朗格汉斯细胞的存在下，进行了评估，使用一个面板的单克隆抗体immunocytochemically.Results重建表皮的所有层正常分化（细胞角蛋白免疫细胞化学）。基底层含有IV型胶原和层粘连蛋白。重建的表皮广泛地由朗格汉斯细胞定植。结论所描述的技术的结果是令人鼓舞的，特别是在烧伤后的患者。所述方法适用于临床实践中皮肤缺损的治疗。
Background Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers.Methods Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies.Results All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells.Conclusions The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.