Re-evaluation of the effects of neuropeptide Y on aggregation of human platelets.
Re-evaluation of the effects of neuropeptide Y on aggregation of human platelets.
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重新评估神经肽 Y 对人血小板聚集的影响。
DOI：
10.1016/0024-3205(91)90072-j
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发表时间：
1991
期刊：
影响因子：
6.1
通讯作者：
Zukowska-Grojec,Z
中科院分区：
文献类型：
--
作者：
Myers,AK;Abi-Younes,S;Zukowska-Grojec,Z
几项研究表明，血小板是大鼠循环神经肽Y（NPY）免疫反应性的主要来源，但这种血管收缩肽对血小板的影响尚未明确。最近，据报道，猪NPY是肾上腺素诱导的体外人血小板聚集的抑制剂，这一观察结果对涉及血小板活化和血栓形成的状态期间的血小板-血管相互作用具有重要意义。因此，我们进行了目前的研究，试图证实早期的报告，并将这些观察结果扩展到人类NPY。与最近的报道相反，我们发现人或猪的NPY对肾上腺素或胶原诱导的正常人血小板聚集没有抑制作用。同样，特异性NPY Y-1和Y-2激动剂对血小板聚集没有直接或间接作用。最后，测定人NPY对血小板内cAMP的影响。该肽对基础或伊洛前列素刺激的cAMP水平均无影响。我们推测NPY在血小板-血管相互作用中的作用是促进与血小板聚集相关的血管收缩，而不包括抑制进一步的血栓形成。
Several studies have shown that platelets are a major source of circulating neuropeptide Y (NPY) immunoreactivity in rats, but the effects of this vasoconstrictor peptide on platelets are not well-defined. Recently, it was reported that porcine NPY was an inhibitor of in vitro human platelet aggregation induced by epinephrine, an observation which would have important implications regarding platelet-vascular interactions during states involving platelet activation and thrombosis. Thus, we undertook the present studies, in an attempt to confirm the earlier report, and to extend those observations to human NPY. In contrast to the recent report, we found no inhibitory effect of either human or porcine NPY on epinephrine-or collagen-induced aggregation of human platelets from normal subjects. Likewise, specific NPY Y-1 and Y-2 agonists had no direct or indirect action on platelet aggregation. Finally, the effect of human NPY on intraplatelet cAMP was measured. The peptide had no effect on either basal or iloprost-stimulated cAMP levels. We hypothesize that the role of NPY in the platelet-vascular interaction is in promoting vasoconstriction associated with platelet aggregation, and does not include inhibition of further thrombosis.