Desensitization of beta-adrenergic receptors in corneal endothelium.
Desensitization of beta-adrenergic receptors in corneal endothelium.
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角膜内皮β-肾上腺素能受体脱敏。
DOI：
10.1089/jop.1985.1.263
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Walkenbach,RJ
中科院分区：
文献类型：
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作者：
Chao,WT;Walkenbach,RJ
原代培养的角膜内皮细胞对异丙肾上腺素（Iso）、肾上腺素（Epi）、去甲肾上腺素（NE）、前列腺素E1和E2（PGE 1、PGE 2）和毛喉素的反应是，在10分钟的孵育期内，环磷酸腺苷分别增加到对照组的61、52、28、14、10和176倍。然而，当细胞与Iso预孵育1小时时，它们对随后的Iso攻击失去了76%的反应性。Iso预孵育也降低了其他β-肾上腺素能激动剂（Epi和NE）的效应，但对PGE 1、PGE 2和forskolin的效应保持不变。环AMP反应的降低与β-肾上腺素能结合位点的丧失有关。通过普萘洛尔置换[125 I]吲哚洛尔结合测定，其余受体的亲和力不变。这项研究表明，角膜内皮细胞不仅对β-肾上腺素能刺激有反应，而且还可以调节其对激素反应的幅度和持续时间。
Corneal endothelial cells in primary culture responded to isoproterenol (Iso), epinephrine (Epi), nor-epinephrine (NE), Prostaglandins E1and E2(PGE1, PGE2) and forskolin by increasing cyclic AMP 61, 52, 28, 14, 10, and 176-fold, respectively,over control within a 10 minute incubation period. However, when cells were preincubated with Iso for one hour, they lost 76% of their responsiveness to a subsequent Iso challenge. Iso preincubation also decreased the effectiveness of other β-adrenergic agonists (Epi and NE), but responses to PGE1, PGE2and forskolin remained unchanged. The decreased cyclic AMP response was associated with a loss of β-adrenergic binding sites. The affinity of the remaining receptors was unchanged as determined by propranolol displacement of [125I ] pindolol binding. This study shows that corneal endothelial cells not only respond to β-adrenergic stimuli, but can also regulate the magnitude and duration of their response to the hormone.