Prognostic significance of Id-1 and its association with EGFR in renal cell cancer
Prognostic significance of Id-1 and its association with EGFR in renal cell cancer
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DOI：
10.1111/j.1365-2559.2007.02637.x
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发表时间：
2007-03-01
期刊：
影响因子：
6.4
通讯作者：
Wang, X.
中科院分区：
文献类型：
--
作者：
Li, X.;Zhang, Z.;Wang, X.
目的：表皮生长因子受体（EGFR）是肾细胞癌（RCC）侵袭性进展的正调控因子之一。最近，Id-1（分化或DNA结合抑制剂），螺旋-环-螺旋转录因子，已被确定为EGFR信号通路的关键因子之一。为探讨Id-1在肾癌组织中的表达及其与EGFR表达的关系，采用免疫组织化学方法检测107例肾癌组织芯片（TMA）和32例正常肾组织中Id-1和EGFR的表达。相对Id-1和EGFR蛋白表达通过估计染色强度在四级量表上进行定量。我们发现，虽然在非恶性肾组织中观察到Id-1和EGFR的阴性至弱表达，但大多数RCC在肿瘤细胞中显示出显著的Id-1和EGFR阳性表达。此外，Id-1免疫染色强度与肿瘤分期、分级和EGFR表达呈正相关。结论：Id-1过表达是晚期肾癌的一个新的标志物，与EGFR表达呈正相关。我们的研究结果表明，Id-1可能在肾细胞癌的发展中发挥重要作用，并表明Id-1是一个潜在的预后不良的患者的标志物。
Aims: Epidermal growth factor receptor (EGFR) is suggested as one of the positive regulators in the invasive progression of renal cell cancer (RCC). Recently, Id-1 (inhibitor of differentiation or DNA binding), a helix-loop-helix transcription factor, has been identified as one of the key factors in the EGFR signalling pathway. The aim of this study was to investigate the significance of Id-1 expression in renal cell cancer and to study its relationship with EGFR.Methods and results: Id-1 and EGFR expression was examined in tissue microarray (TMA) samples of 107 RCC and 32 normal kidney specimens by immunohistochemistry. Relative Id-1 and EGFR protein expression was quantified by estimating the staining intensity on a four-grade scale. We found that while negative to weak expression of Id-1 and EGFR was observed in non-malignant kidney tissues, most RCCs showed significant positive Id-1 and EGFR expression in tumour cells. In addition, Id-1 immunostaining intensity was positively associated with increased tumour staging, grading and EGFR expression.Conclusions: Overexpression of Id-1 is a novel marker for advanced RCC which is positively correlated with EGFR expression. Our results suggest that Id-1 may play an important role in the development of RCC and indicate that Id-1 is a potential marker of patients with a poor prognosis.