Unlocking the secrets of the microbiome: exploring the dynamic microbial interplay with humans through metabolomics and their manipulation for synthetic biology applications.
Unlocking the secrets of the microbiome: exploring the dynamic microbial interplay with humans through metabolomics and their manipulation for synthetic biology applications.
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DOI：
10.1042/bcj20210534
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发表时间：
2023-06-28
影响因子：
4.1
通讯作者：
Goodacre, Royston
中科院分区：
文献类型：
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作者：
Muhamadali, Howbeer;Winder, Catherine L.;Dunn, Warwick B.;Goodacre, Royston
代谢组学是一种强大的研究发现工具，具有测量数百至数千种代谢物的潜力。在这篇综述中，我们讨论了GC-MS和LC-MS在基于发现的代谢组学研究中的应用，我们定义了代谢组学工作流程，并强调了需要解决的问题，以生成稳健和可重复的数据。我们强调，代谢组学现在通常应用于整个生物科学，研究微生物组，从相对简单的微生物系统到宿主和环境中财团内的复杂相互作用，并在一系列生物物种和哺乳动物系统（包括人类）中突出这一点。然而，仍然存在需要克服的挑战，以最大限度地发挥代谢组学的潜力，帮助我们了解生物系统。为了证明这种方法的潜力，我们讨论了代谢组学在两个广泛的研究领域中的应用：（1）合成生物学，以增加高价值精细化学品的生产并减少次级副产物;（2）肠道微生物与人类宿主的相互作用。虽然后者的重要性正在迅速增加，但仍处于起步阶段，将受益于开发工具来解决宿主-肠道-微生物相互作用及其对人类健康和疾病的影响。
Metabolomics is a powerful research discovery tool with the potential to measure hundreds to low thousands of metabolites. In this review, we discuss the application of GC–MS and LC–MS in discovery-based metabolomics research, we define metabolomics workflows and we highlight considerations that need to be addressed in order to generate robust and reproducible data. We stress that metabolomics is now routinely applied across the biological sciences to study microbiomes from relatively simple microbial systems to their complex interactions within consortia in the host and the environment and highlight this in a range of biological species and mammalian systems including humans. However, challenges do still exist that need to be overcome to maximise the potential for metabolomics to help us understanding biological systems. To demonstrate the potential of the approach we discuss the application of metabolomics in two broad research areas: (1) synthetic biology to increase the production of high-value fine chemicals and reduction in secondary by-products and (2) gut microbial interaction with the human host. While burgeoning in importance, the latter is still in its infancy and will benefit from the development of tools to detangle host–gut-microbial interactions and their impact on human health and diseases.