In vitro production of biofilm in a flow cell system in a strain of Pseudomonas aeruginosa and Staphylococcus aureus and determination of efficiency of ciprofloxacin against them.
In vitro production of biofilm in a flow cell system in a strain of Pseudomonas aeruginosa and Staphylococcus aureus and determination of efficiency of ciprofloxacin against them.
复制标题
铜绿假单胞菌和金黄色葡萄球菌菌株的流动细胞系统中生物膜的体外生产以及环丙沙星对抗它们的效率的测定。
DOI：
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发表时间：
2011
影响因子：
1
通讯作者：
S. Muralidharan
中科院分区：
文献类型：
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作者：
Soham Gupta;S. Agarwal;D. Sahoo;S. Muralidharan
背景
微生物在各种医疗器械上形成生物膜。该过程在公共卫生中特别相关，因为生物膜相关生物体对抗生素的耐药性更强，并且有可能导致留置医疗器械的患者感染。
材料和方法
为了确定抗生素对生物被膜的效率，不适合使用确定自由漂浮实验室表型的最小抑制浓度（MIC）的传统技术。因此，我们已经诱导形成的生物被膜中的两个菌株（铜绿假单胞菌和金黄色葡萄球菌，这表明在通过管法筛选的生物被膜的重生长）在流动池系统中，并确定其对环丙沙星的抗生素敏感性通过琼脂稀释法在范围内（0.25 mg/ml至8 mg/ml）。将环丙沙星对生物膜产生的生物体的MIC值与其游离形式和作为对照的标准菌株在相同平板上进行比较。
意见
两种生物膜产生菌的MIC均高于游离菌，铜绿假单胞菌为2 μg/ml，金黄色葡萄球菌为4 mg/ml。因此，生物膜可能对患者治疗构成威胁。
BACKGROUND
Microorganisms develop biofilm on various medical devices. The process is particularly relevant in public health since biofilm associated organisms are much more resistant to antibiotics and have a potential to cause infections in patients with indwelling medical devices.
MATERIALS AND METHODS
To determine the efficiency of an antibiotic against the biofilm it is inappropriate to use traditional technique of determining Minimum Inhibitory Concentration (MIC) on the free floating laboratory phenotype. Thus we have induced formation of biofilm in two strains (Pseudomonas aeruginosa and Staphylococcus aureus, which showed heavy growth of biofilm in screening by Tube method) in a flow cell system and determined their antibiotic susceptibility against ciprofloxacin by agar dilution method in the range (0.25 mg/ml to 8 mg/ml). The MIC value of ciprofloxacin for the biofilm produced organism was compared with its free form and a standard strain as control on the same plates.
OBSERVATIONS
Both the biofilm produced strains showed a higher resistance (MIC > 8 mg/ml) than its free form, which were 2 μg/ml for Pseudomonas aeruginosa and 4 mg/ml for Staphylococcus aureus. Thus biofilm can pose a threat in the patient treatment.