Discovery and characterization of an epithelial-specific galectin in the endometrium that forms crystals in the trophectoderm
Discovery and characterization of an epithelial-specific galectin in the endometrium that forms crystals in the trophectoderm
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DOI：
10.1073/pnas.0402669101
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发表时间：
2004-05-25
影响因子：
11.1
通讯作者：
Spencer, TE
中科院分区：
文献类型：
--
作者：
Gray, CA;Adelson, DL;Spencer, TE
在怀孕期间，子宫的分泌物支持胚胎(胚胎/胎儿和相关的膜)的存活和生长。Galectin-15，也被称为OVGAL11，是在绵羊子宫中发现的Galectin家族的一个未知成员，该家族分泌的β-Galactoside凝集素含有一个保守的碳水化合物识别结构域和一个独立的整合素结合结构域。在周期绵羊和妊娠绵羊的子宫内膜中，Galectin-15mRNA在子宫内膜腔上皮细胞中特异表达，而在胚胎中不表达。妊娠绵羊子宫内膜上皮细胞在第10~12天出现Galectin-15mRNA的表达，第12~16天表达增加。孕酮诱导和干扰素-tau刺激子宫内膜上皮Galectin-15mRNA的表达。Galectin-15蛋白主要集中在子宫内膜腔上皮细胞顶面附近，并定位于滋养外胚层离散的胞质结晶结构中。在子宫腔内，分泌的Galectin-15蛋白在怀孕14天至16天期间增加。Galectin-15蛋白具有结合乳糖和甘露糖的功能，在免疫学上与绵羊子宫中未命名的M-r14000(14K)蛋白相同，后者在子宫内膜上皮和受孕Tr中形成结晶包涵体。基于其他Galectins的功能研究，Galectin-15被认为在细胞外调节Tr的迁移和与子宫内膜上皮的黏附，并在细胞内调节Tr细胞的存活、生长和分化。Galectins可作为子宫内膜功能和容受性的细胞和分子标记物，促进胚胎存活和发育，以及评估和提高生育能力。
Secretions of the uterus support survival and growth of the conceptus (embryo/fetus and associated membranes) during pregnancy. Galectin-15, also known as OVGAL11 and a previously uncharacterized member of the galectin family of secreted beta-galactoside lectins containing a conserved carbohydrate recognition domain and a separate putative integrin binding domain, was discovered in the uterus of sheep. In endometria of cyclic and pregnant sheep, galectin-15 mRNA was expressed specifically in the endometrial luminal epithelium but not in the conceptus. In pregnant sheep, galectin-15 mRNA expression appeared in the epithelia between days 10 and 12 and increased between days 12 and 16. Progesterone induced and IFN-tau stimulated galectin-15 mRNA in the endometrial epithelium. Galectin-15 protein was concentrated near and on the apical surface of the endometrial luminal epithelia and localized within discrete cytoplasmic crystalline structures of conceptus trophectoderm (Tr). In the uterine lumen, secreted galectin-15 protein increased between days 14 and 16 of pregnancy. Galectin-15 protein was functional in binding lactose and mannose sugars and immunologically identical to the unnamed M-r 14,000 (14K) protein from the ovine uterus that forms crystalline inclusion bodies in endometrial epithelia and conceptus Tr. Based on the functional studies of other galectins, galectin-15 is hypothesized to function extracellularly to regulate Tr migration and adhesion to the endometrial epithelium and intracellularly to regulate Tr cell survival, growth, and differentiation. Galectins may be useful as cellular and molecular markers for endometrial function and receptivity, to enhance conceptus survival and development, and to evaluate and enhance fertility.