The existence of ferric hydroxide links the carbon and nitrogen cycles by promoting nitrite-coupled methane anaerobic oxidation.
The existence of ferric hydroxide links the carbon and nitrogen cycles by promoting nitrite-coupled methane anaerobic oxidation.
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DOI：
10.1016/j.watres.2023.120192
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发表时间：
2023-06
期刊：
影响因子：
12.8
通讯作者：
Juan Huang;Wurong Zhao;Jinwei Ju;Suifen Liu;Jinshao Ye;Yan Long
中科院分区：
文献类型：
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作者：
Juan Huang;Wurong Zhao;Jinwei Ju;Suifen Liu;Jinshao Ye;Yan Long
微生物介导的甲烷厌氧氧化可以有效地减少甲烷的大气排放，是连接碳、氮和铁的生态地球化学循环的关键过程。结果表明，与对照相比，活性炭对甲烷氧化和亚硝酸盐的去除率分别提高了1.12倍和1.28倍，表明氢氧化铁对亚硝酸盐驱动的AOM具有增强作用。甲烷菌、甲烷氧化菌和硝化菌的富集是该生化过程的主要媒介，甲烷菌和甲烷氧化菌与Fe ~（3+）、Fe ~（2+）呈正相关，甲基孢囊菌和甲基假单胞菌与甲烷呈正相关，硝化菌与亚硝酸盐呈正相关。宏基因组学分析显示，甲烷氧化、氮还原和血红素C型细胞色素相关基因在CF中表达上调，表明细菌和产甲烷菌的协同作用通过不同的代谢途径驱动AOM，其中氢氧化铁起着至关重要的作用。该研究为三价铁和亚硝酸盐驱动的AOM协同机制提供了新的见解。
Microorganism-mediated anaerobic oxidation of methane can efficiently mitigate methane atmospheric emissions and is a key process linking the biogeochemical cycles of carbon, nitrogen, and iron. The results showed that methane oxidation and nitrite removal rates in the CF were 1.12 and 1.28 times higher than those in CK, respectively, suggesting that ferric hydroxide can enhance nitrite-driven AOM. The biochemical process was mediated by the enrichment of methanogens, methanotrophs, and denitrifiers.MethanobacteriumandMethanosarcinawere positively correlated with Fe3+and Fe2+, whereasMethylocystisandMethylocaldumwere positively correlated with methane, and denitrifiers were positively correlated with nitrite. Metagenomic analysis revealed that the genes related to methane oxidation, nitrogen reduction, and heme c-type cytochrome were upregulated in CF, indicating that a synergistic action of bacteria and methanogens drove AOM via diverse metabolic pathways, within which ferric hydroxide played a crucial role. This study provides novel insights into the synergistic mechanism of ferric iron and nitrite-driven AOM.