Determination of soyasaponins in soy with LC-MS following structural unification by partial alkaline degradation
Determination of soyasaponins in soy with LC-MS following structural unification by partial alkaline degradation
复制标题
DOI：
10.1021/jf0257300
复制
发表时间：
2002-11-20
影响因子：
6.1
通讯作者：
Prior, RL
中科院分区：
文献类型：
--
作者：
Gu, LW;Tao, GJ;Prior, RL
采用高效液相色谱-电喷雾质谱联用技术对大豆中的大豆皂苷进行了研究。发现属于A组的每种大豆皂苷主要以其真正的乙酰化形式存在。部分到完全脱乙酰化的结构以不同的比例共存。同样，检测到属于Bin大豆的大豆皂苷。作为2，3-二氢-2，5-二羟基-6-甲基-4-吡喃酮（D-吡喃酮）共轭形式和非D-吡喃酮形式。大豆皂甙结构的多样性阻碍了大豆皂甙的生物活性。分离和测定大豆中的单个化合物。在本研究中，从大豆中提取的大豆皂苷在温和的条件下用氢氧化钠处理，以切断A组大豆皂苷中的乙酰基和组大豆皂苷中的二硫键。B;而糖苷结构不受影响。通过这样做，所有的大豆皂苷从相同的初始结构被统一到定义明确的结构，然后单独定量，使用其[M-H]（-）离子的选择性离子记录。以脱乙酰和非脱乙酰大豆皂甙为外标。A组和B组大豆皂苷的定量限分别为1.74和1.89 ng，回收率分别为94.1% ± 4.2%和96.9% ± 2.9%。
High-performance liquid chromatography coupled With electrospray ionization mass spectrometer was used to study the soyasaponins in soy. It was found that each soyasaponin belonging to group A existed mainly in their genuine acetylated forms. The partially to fully deacetylated structures coexisted in various proportions. Likewise, the soyasaponins belonging to group Bin soy were detected.. as both 2,3-dihydro-2,5-dihydroxy-6-methyl-4-pyrone (DDMP) conjugated forms and non-DDMP forms. The structural diversity of soyasaponins hinders the. separation and determination of the individual compounds in soy. In the present studies, the soyasaponins extracted from soy were treated with sodium hydroxide under mild conditions to cleave the acetyl groups from soyasaponins in group A as well as the DDMP from soyasaponins in group. B; while the glycoside structures remained unaffected. By doing so, all soyasaponins originating from the same initial structures were unified into well-defined structures and then quantified individually, using the selective ion recording of their [M-H](-) ions. The pure deacetyl and non-DDMP soyasaponins were used as the external standards. The quantification limits of soyasaponins in group A and group B were 1.74 and 1.89 ng injected on column with recovery rates of 94.1% +/- 4.2% and.96.9% +/- 2.9%, respectively.