Species-specific binding of purified pili (AF/R1) from the Escherichia coli RDEC-1 to rabbit intestinal mucosa
Species-specific binding of purified pili (AF/R1) from the Escherichia coli RDEC-1 to rabbit intestinal mucosa
复制标题
大肠杆菌 RDEC-1 纯化菌毛 (AF/R1) 与兔肠粘膜的物种特异性结合
DOI：
10.1016/0016-5085(83)90433-x
复制
发表时间：
1983
期刊：
影响因子：
29.4
通讯作者：
E. Boedeker
中科院分区：
文献类型：
--
作者：
R. Berendson;C. Cheney;P. A. Schad;E. Boedeker
RDEC-1大肠埃希菌在兔肠道的种属特异性定植是一种公认的细菌粘膜黏附的动物模型。为了确定RDEC-1菌毛是否具有黏附因子的功能，我们在促进菌毛表达的条件下培养了菌毛；我们分离了菌毛，记录了它们的纯度，并使用间接免疫荧光技术比较了它们的粘膜黏附特性与整个生物体的黏附特性。将兔、大鼠、豚鼠和人的小肠冰冻切片分别与去毛或去毛的RDEC-1菌毛或纯化的RDEC-1菌毛孵育，观察0~4+的荧光分布和强度。被纤毛的RDEC-1生物发出明亮的荧光(4+)，分布在兔回肠的整个粘膜表面。只有几个无毛的RDEC-1附着在兔回肠上，它们随机分布在整个组织切片上。用纯RDEC-1菌毛覆盖的兔回肠在从隐窝到绒毛顶端的粘膜表面显示出特异性的抗d-甘露糖(2-3+)荧光。在覆盖有RDEC-1菌毛的豚鼠、大鼠或人的粘膜表面未见荧光。纯化的RDEC-1菌毛以一种特定的方式附着在兔肠黏膜上，并与整个有毛的生物具有相同的分布。这些数据表明，RDEC-1产生菌毛(与1型菌毛不同)，这决定了RDEC-1与兔回肠粘膜粘连的特异性。
Species-specific colonization of rabbit intestine by RDEC-1Escherichia coliis an accepted animal model for bacterial mucosal adherence. To determine whether RDEC-1 pili are functional as adherence factors for this organism, we grew the organism under conditions that promoted pilus expression; we isolated the pili, documented their purity, and compared their mucosal adherence properties with those of whole organisms using an indirect immunofluorescence technique. Frozen sections of rabbit, rat, guinea pig, and human small intestine were incubated with either piliated or nonpiliated RDEC-1 organisms or purified RDEC-1 pili and observed for the distribution and intensity (0–4+) of fluorescence. Piliated RDEC-1 organisms fluoresced brightly (4+) and were distributed along the entire mucosal surface of the rabbit ileum. Only a few nonpiliated RDEC-1 attached to rabbit ileum, and they were randomly scattered across the entire section of tissue. Rabbit ileum overlain with pure RDEC-1 pili showed a specific,d-mannose resistant (2–3+) fluorescence on the mucosal surface from the crypts to the villus tips. No fluorescence was seen on the guinea pig, rat, or human mucosal surface overlain with RDEC-1 pili. Purified RDEC-1 pili adhere to the rabbit intestinal mucosa in a species-specific manner and with the same distribution as whole piliated organisms. The data suggest that RDEC-1 produce pili (distinct from type 1 pili) that determine the specificity of the mucosal adherence of RDEC-1 to rabbit ileum.
肠细胞粘附性大肠杆菌的临床病理学研究：婴儿迁延性腹泻的原因。
影响因子：
29.4
作者：
Rothbaum,R;McAdams,AJ;Giannella,R;Partin,JC
通讯作者：
Partin,JC