DNA based cladograms augment the discovery of a new Ips species from China (Coleoptera: Curculionidae: Scolytinae)
DNA based cladograms augment the discovery of a new Ips species from China (Coleoptera: Curculionidae: Scolytinae)
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DOI：
10.1111/j.1096-0031.2007.00159.x
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发表时间：
2007-12-01
期刊：
影响因子：
3.6
通讯作者：
Sun, Jiang Hua
中科院分区：
文献类型：
--
作者：
Cognato, Anthony I.;Sun, Jiang Hua
DNA在分类学研究中的应用还处于起步阶段，因为大多数分类群的核苷酸变化的数量和类型与物种边界的关系还没有得到充分的研究。线粒体细胞色素c氧化酶I（COI）核苷酸数据是目前最流行的分子标记物，用于划定物种边界和一个标准的成对的核苷酸差异群体之间的个人已被建议识别新的物种。这是不太可能的，这样的标准将适用于整个动物物种，但协会的数量和类型的核苷酸变化与物种边界可能有助于建立一个分类单元特异性的DNA taxonomy.This研究利用DNA数据从核和线粒体基因，以提高一个重要的森林甲虫害虫，Ips的分类。基于这些数据的分支分析，核苷酸差异的数量和类型与单系种相关。因此，一个新的物种从中国描述的甲虫的进化枝，其核苷酸差异超过了目前公认的物种内观察到的进化变化量。COI数据独立分析扩展的分类单元数据集，包括成对的识别的姐妹物种之间的核苷酸差异。种内平均成对核苷酸差异的范围很广（0-10.0%），表明将标准核苷酸差异百分比作为识别种间边界的方法存在局限性。最多，平均COI核苷酸种内差异提供了一个非正式的指导，以确定潜在的分支，可能需要进一步的系统调查。(C)2007年，威利·亨尼格协会。
The implementation of DNA in taxonomic study is in its infancy because the association of the amount and type of nucleotide change with species boundaries has not been fully examined for most taxa. Mitochondrial cytochrome c oxidase I (COI) nucleotide data is currently the most popular molecular marker for delimiting species boundaries and a standard pair-wise nucleotide divergence between groups of individuals has been suggested for the recognition of new species. It is unlikely that such a standard would be applicable across animal species, but the association of the amount and type of nucleotide change with species boundaries could help with the establishment of a taxon-specific DNA taxonomy.This study utilizes DNA data from nuclear and mitochondrial genes to improve the taxonomy of an important forest beetle pest, Ips. Amount and type of nucleotide difference are associated with monophyletic species based on a cladistic analysis of these data. As a result, a new species from China is described for a clade of beetles whose nucleotide differences exceeded the amount of evolutionary change observed within currently recognized species. The COI data are analyzed independently with an expanded taxon data set, including pair-wise nucleotide differences between recognized sister species. The wide range of average intraspecific pair-wise nucleotide difference (0-10.0%) suggests limitations to the application of a standard percent nucleotide difference as a means to identify species boundaries. At most, average COI nucleotide intraspecific difference provides an informal guide to identify potential clades that may warrant further systematic investigation. (C) The Willi Hennig Society 2007.