Expression and co-expression of VR1, CGRP, and IB4-binding glycoprotein in dorsal root ganglion neurons in rats: Differences between the disc afferents and the cutaneous afferents
Expression and co-expression of VR1, CGRP, and IB4-binding glycoprotein in dorsal root ganglion neurons in rats: Differences between the disc afferents and the cutaneous afferents
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DOI：
10.1097/01.brs.0000167532.96540.31
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发表时间：
2005-07-01
期刊：
影响因子：
3
通讯作者：
Moriya, H
中科院分区：
文献类型：
--
作者：
Aoki, Y;Ohtori, S;Moriya, H
研究设计.香草酸受体1（vanilloid receptor 1，VR 1）、降钙素基因相关肽（calcitonin gene-related peptide，CGRP）和异凝集素B4（isolectin B4，IB 4）结合糖蛋白在支配腰椎间盘和足底皮肤的背根神经节（dorsal root ganglion，DRG）神经元中的表达。背根节小的伤害感受神经元分为神经生长因子（NGF）敏感神经元和胶质细胞源性神经营养因子（GDNF）敏感神经元。CGRP和IB 4结合糖蛋白分别被认为是NGF和GDNF敏感神经元的特异性标志物。VR 1定位于小DRG神经元。采用组织化学染色和逆行追踪方法，观察了大鼠L5-L 6椎间盘和足底皮肤背根节神经元中VR 1、CGRP和IB 4结合糖蛋白的表达。在支配椎间盘的DRG神经元中，VR 1、CGRP和IB 4结合糖蛋白的阳性表达率分别为23.4%、54.4%和1.0%。支配皮肤的DRG神经元的分布如下：35.1%的VR 1，41.1%的CGRP和19.5%的IB 4结合糖蛋白。支配椎间盘的DRG神经元中VR 1和IB 4结合糖蛋白阳性神经元的百分比显著低于支配皮肤的DRG神经元（P < 0.05），而CGRP阳性神经元的百分比差异无显著性。VR 1在腰椎间盘中的含量低于皮肤组织。我们的数据表明，从光盘的伤害性信息主要是由神经生长因子敏感的神经元，而从皮肤组织的神经生长因子敏感和GDNF敏感的神经元传递。
Study Design. The expression of vanilloid receptor 1 (VR1), calcitonin gene-related peptide ( CGRP), and isolectin B4 ( IB4)-binding glycoprotein in dorsal root ganglion (DRG) neurons innervating the lumbar disc and the plantar skin was investigated.Objective To characterize the DRG neurons innervating lumbar discs and those innervating cutaneous tissue in rats.Summary and Background Data. Small nociceptive DRG neurons are divided into nerve growth factor (NGF) sensitive and glial cellline-derived neurotrophic factor (GDNF)-sensitive neurons. CGRP and IB4-binding glycoprotein are recognized as specific markers for NGF and GDNF-sensitive neurons, respectively. VR1 is localized in small DRG neurons.Methods. Using histochemical staining and retrograde tracing methods, the expression of VR1, CGRP, and IB4-binding glycoprotein in DRG neurons innervating the L5-L6 disc and the plantar skin was examined in rats.Results. DRG neurons innervating the disc showed positive staining as: 23.4% VR1, 54.4% CGRP, and 1.0% IB4-binding glycoprotein. The following distribution was found for DRG neurons innervating the skin: 35.1% VR1, 41.1% CGRP, and 19.5% IB4-binding glycoprotein. Percentages of neurons positive for VR1 and IB4-binding glycoprotein were significantly lower in DRG neurons innervating the disc than in DRG neurons innervating the skin (P < 0.05), while no significant difference was observed in the percentage of neurons positive for CGRP.Conclusions. VR1 is less abundant in lumbar disc than in cutaneous tissue. Our data suggest that nociceptive-information from the disc is transmitted mostly by NGF-sensitive neurons, while that from the cutaneous tissue is transmitted by both NGF-sensitive and GDNF-sensitive neurons.