Development of Quantum Dot-Mediated Fluorescence Thermometry for Thermal Therapies
Development of Quantum Dot-Mediated Fluorescence Thermometry for Thermal Therapies
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DOI：
10.1007/s10439-009-9681-6
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发表时间：
2009-06-01
影响因子：
3.8
通讯作者：
Cadeddu, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Han, Bumsoo;Hanson, Willard L.;Cadeddu, Jeffrey A.
由于热疗法经常用于管理各种器官中的肿瘤，因此对热损伤的可靠且准确的术中监测技术的需求日益增长。然而，目前的监测技术具有有限的准确性、可访问性，并且不能在手术期间实时监测热损伤。在本研究中，量子点介导的荧光测温法的发展和其性能的特点，以证明时空监测的热损伤的可行性。首先，两种不同类型的CdTe/ZnS量子点（QD）的温度依赖性的特征在于在相关的热疗的温度范围内，并建立每个QD的温度-强度关系。通过将QD暴露于预定的空间温度梯度，并通过在金纳米壳介导的加热期间监测时空温度，来表征系统的空间和时间分辨率。结果表明，量子点介导的温度测量是能够测量时空变化的温度场相关的热疗。本文还讨论了它在术中热疗图像引导中的意义。
As thermal therapies are frequently employed for management of tumors in various organs, there are growing demands for reliable and accurate intraoperative monitoring techniques of the thermal lesion. However, current monitoring techniques have limited accuracy, accessibility and are not capable of monitoring the thermal lesion in real-time during the procedure. In the present study, quantum dot-mediated fluorescence thermometry was developed and its performance was characterized to demonstrate the feasibility of spatiotemporal monitoring of thermal lesions. First, the temperature dependency of two different types of CdTe/ZnS quantum dots (QDs) were characterized in a temperature range relevant to hyperthermic therapies, and a temperature-intensity relationship was established for each QD. The spatial and temporal resolutions of the system were characterized by exposing QDs to a pre-determined spatial temperature gradient, and by monitoring the spatiotemporal temperature during gold nanoshell-mediated heating. The results demonstrated that QD-mediated thermometry is capable of measuring spatiotemporally varying temperature fields relevant for hyperthermic thermal therapies. Its implication for intraoperative image-guidance of thermal therapy was also discussed.