Serological identification of adult T-cell leukaemia-associated antigens
Serological identification of adult T-cell leukaemia-associated antigens
复制标题
DOI：
10.1111/j.1365-2141.2005.05619.x
复制
发表时间：
2005-08-01
影响因子：
6.5
通讯作者：
Uchiyama, T
中科院分区：
文献类型：
--
作者：
Hishizawa, M;Imada, K;Uchiyama, T
成人T细胞白血病（ATL）是由人T细胞白血病病毒I型（HTLV-I）引起的外周T细胞肿瘤。一些临床观察表明，ATL中的一些肿瘤相关抗原可能被免疫系统识别。在这项研究中，我们进行了血清学筛选的表达文库，以确定ATL相关抗原，使用材料从一个独特的ATL患者的长期稳定的疾病。在5个不同的基因分离，丝氨酸/精氨酸蛋白激酶1（SRPK 1），这已被报道有一个有限的正常组织分布，被发现在大多数急性型ATL样品中过表达，但在慢性型ATL或正常外周血单核细胞的实时逆转录聚合酶链反应。有趣的是，SRPK 1在侵袭性ATL中的过表达在蛋白质水平上比在mRNA水平上更特异性地观察到。使用血浆蛋白质印迹分析证实ATL患者中存在SRPK 1自身抗体，但在无症状HTLV-I携带者或健康志愿者中未检测到。这些结果表明，SRPK 1可能是有用的发展与ATL患者的治疗和诊断方法。
Adult T-cell leukaemia (ATL) is a peripheral T-cell neoplasm caused by human T-cell leukaemia virus type I (HTLV-I). Several clinical observations suggest that some tumour-associated antigens in ATL may be recognised by the immune system. In this study, we performed the serological screening of an expression library to identify ATL-associated antigens by using materials from a unique ATL patient with long-term stable disease. Among five distinct genes isolated, serine/arginine protein kinase 1 (SRPK1), which has been reported to have a restricted normal tissue distribution, was found to be overexpressed in most acute type ATL samples, but not in chronic type ATL or in normal peripheral blood mononuclear cells by real-time reverse transcription polymerase chain reaction. Interestingly, the overexpression of SRPK1 in aggressive types of ATL was more exclusively observed at the protein level than at the mRNA level. Autologous antibody to SRPK1 was confirmed in the ATL patient using Western blot analysis with plasma, but not detected in asymptomatic HTLV-I carriers or in healthy volunteers. These results indicate that SRPK1 may be useful for the development of therapeutic and diagnostic methods for patients with ATL.