The evolutionarily highly conserved MST2 suppresses WSSV infection by promoting apoptosis in shrimp
The evolutionarily highly conserved MST2 suppresses WSSV infection by promoting apoptosis in shrimp
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进化上高度保守的MST2通过促进虾细胞凋亡来抑制WSSV感染
DOI：
10.1016/j.aqrep.2022.101296
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发表时间：
2022-08-12
影响因子：
3.7
通讯作者：
Cui，Yalei
中科院分区：
文献类型：
--
作者：
Xu，Junying;Li，Fen;Cui，Yalei
哺乳动物Ste20样激酶2（MST2）是Hippo通路的核心成分，在调节细胞凋亡、细胞增殖和细胞周期进程中发挥重要作用，但在无脊椎动物，尤其是甲壳类动物中的研究很少。在本研究中，对日本对虾的MST2基因进行了表征。获得了编码 690 个氨基酸的 2111 bp 长核苷酸序列。 MST2 蛋白的分子量为 78.47222 kDa。生物信息学分析表明，MST2蛋白具有S_TK-c（Ser/Thr激酶）结构域和SARAH（Salvador/Ras关联结构域家族/Hippo）结构域，这与其他物种一致。亚细胞定位分析表明MST2主要存在于细胞质中。实时PCR和细胞凋亡分析表明MST2参与了白斑综合征病毒（WSSV）感染过程，并通过促进细胞凋亡来抑制病毒感染。该研究揭示了MST2在病毒侵袭和细胞凋亡中的潜在功能，为预防虾病毒感染提供了新的靶点。
Mammalian Ste20-like kinase 2 (MST2) is a core component of the Hippo pathway and plays an important role in regulating cell apoptosis, cell proliferation and cell cycle progression, but it has rarely been studied in invertebrates, especially crustaceans. In this study, theMST2gene inpenaeus japonicusshrimp was characterized. A 2111 bp long nucleotide sequence encoding 690 amino acids was obtained. The molecular weight of MST2 protein is 78.47222 kDa. The bioinformatics analysis indicated that the MST2 protein has an S_TK-c (Ser/Thr kinase) domain and a SARAH (Salvador/Ras-association domain family/Hippo) domain, which is consistent with other species. The analysis of subcellular localization showed that MST2 was mainly present in the cytoplasm. Real time PCR and apoptosis analysis indicated thatMST2was involved in the white spot syndrome virus (WSSV) infection process and inhibited viral infection by promoting apoptosis. This study revealed the potential function ofMST2in virus invasion and cell apoptosis, providing a new target for preventing shrimp virus infection.