1H NMR-based urine metabolic profile of IUGR, LGA, and AGA newborns in the first week of life
1H NMR-based urine metabolic profile of IUGR, LGA, and AGA newborns in the first week of life
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DOI：
10.1016/j.cca.2015.08.008
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发表时间：
2015-12-07
影响因子：
5
通讯作者：
Fanos, Vassilios
中科院分区：
文献类型：
--
作者：
Marincola, Flaminia Cesare;Dessi, Angelica;Fanos, Vassilios
在非最佳营养供应条件下，孕期母体饮食可改变胎儿合成代谢途径和分解代谢途径之间的平衡，触发对代谢综合征的程序化效应。代谢组学是一种分析技术，近年来吸引了越来越多的兴趣来识别饮食暴露的生物标志物。本研究采用基于核磁共振的代谢组学方法，对接受不同胎儿营养的三组新生儿的尿液代谢谱进行了探索性分析，这三组新生儿分别为适合胎龄(AGA)、宫内发育迟缓(IUGR)和较大胎龄(LGA)。尿样是在婴儿出生后的第一周采集的。应用正交偏最小二乘判别分析(OPLS-DA)证明，这三类婴儿的代谢谱随时间的变化相似，主要包括牛磺酸、肌酸、甜菜碱和甘氨酸水平的变化。此外，柠檬酸和肌醇含量的变化是IUGR和LGA的特征，总体水平高于对照组，而β-MINE和琥珀酸的含量在AGA组显著高于对照组。我们的结果积极地支持了代谢组学方法在研究与胎儿营养不良相关的代谢途径方面的应用。(C)2015爱思唯尔B.V.保留所有权利。
Under conditions of non-optimal supply of nutrients, maternal diet during gestation can alter the balance between anabolic and catabolic pathways of fetus and triggers an effect of programming to the metabolic syndrome. Metabolomics is an analytical technique that has been recently attracting increasing interest for the identification of biomarkers of dietary exposure. In this study, a NMR-based metabolomic approach was employed for an explorative analysis of the time-related urinary metabolic profiles of three groups of newborns receiving a different fetal nutrition: adequate for gestational age (AGA), with intrauterine growth retardation (IUGR), and large for gestational age (LGA). Urine samples were collected over the first week of life. Application of Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) evidenced similar time-related modifications in the metabolic profiles of the three classes of infants, consisting mainly of changes in levels of taurine, creatinine, betaine, and glycine. Furthermore, alterations in the content of citrate and myo-inositol were found to be characteristic of IUGR and LGA, whole levels were higher with respect to controls, while higher contents of beta-Mine and succinate were noted in AGA. Our results positively support the application of the metabolomic approach in the study of the metabolic pathways associated to fetal malnutrition. (C) 2015 Elsevier B.V. All rights reserved.