In vivo imaging of spin-trapped nitric oxide in rats with septic shock: MRI spin trapping
In vivo imaging of spin-trapped nitric oxide in rats with septic shock: MRI spin trapping
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DOI：
10.1002/(sici)1522-2594(199908)42:2
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发表时间：
1999-08-01
影响因子：
3.3
通讯作者：
Yoshikawa, K
中科院分区：
文献类型：
--
作者：
Fujii, H;Wan, XM;Yoshikawa, K
本文报道了第一个在体内的核磁共振图像的分布NO使用“MRI自旋捕获”技术。NO与Fe（II）螯合物自旋捕获剂N-甲基-D-葡糖胺二硫代氨基甲酸酯（MGD）络合，通过EPR验证为（MGD）（2）-Fe（II）-NO，并且通过MR图像“可视化”自由基分布。在大鼠中，（MGD）（2）-Fe（II）-NO复合物集中在肝脏中，显示肝静脉和下腔静脉等血管结构的显着增强对比。通过预先给予竞争性抑制剂N-单甲基-L-精氨酸，证实一氧化氮合酶是脓毒性休克大鼠NO的来源，导致增强作用减弱。与其他稳定的含氮自由基（如氮氧自由基）相比，NO络合物在体内更稳定，是一种更有效的MRI造影剂。当与适当的自由基络合剂（如（MGD）（2）-Fe（II））结合使用时，MRI自旋捕获方法应该是一种用于可视化病理器官和组织中自由基空间分布的有力工具。Magn Reson Med 42：235-239，1999. (C)1999 Wiley-Liss，Inc.
This paper reports the first in vivo NMR image of the distribution of NO using the "MRI spin-trapping" technique. NO was complexed with the Fe(II)-chelate spin trap, N-methyl-D-glucamine dithiocarbamate (MGD), verified as (MGD)(2)-Fe(II)-NO by EPR, and the radical distribution was "visualized" by MR images. In rats, the (MGD)(2)-Fe(II)-NO complex was concentrated in the liver displaying significantly enhanced contrast in the vascular structure such as hepatic vein and inferior vena cava. Nitric oxide synthase was verified as the source of NO in rats with septic shock by pre-administration of the competitive inhibitor N-monomethyl-L-arginine, resulting in reduced enhancement. The NO complex was more stable in vivo and a more effective MRI contrast agent than other stable nitrogen containing radicals, such as nitroxides, The MRI spin-trapping method should be a powerful tool for visualizing spatial distributions of free radicals in pathologic organs and tissues when combined with the appropriate radical complexing agent, such as (MGD)(2)-Fe(II) used in these studies. Magn Reson Med 42:235-239, 1999. (C) 1999 Wiley-Liss, Inc.