PAS‐lead hematoxylin as a stain for small‐granule endocrine cell populations in the lungs, other pharyngeal derivatives and the gut
PAS‐lead hematoxylin as a stain for small‐granule endocrine cell populations in the lungs, other pharyngeal derivatives and the gut
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PAS-铅苏木精作为肺部、其他咽部衍生物和肠道中小颗粒内分泌细胞群的染色剂
DOI：
10.1002/ar.1091920205
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
R. Hoyt
中科院分区：
文献类型：
--
作者：
S. Sorokin;R. Hoyt
构成呼吸系统小颗粒细胞群的几种亚型上皮细胞的研究很少，部分原因是与在肠道和其他具有弥漫性内分泌系统的器官中相比，很难获得足够的银、单胺荧光和其他特定的光显微镜制剂。周期性酸-希夫（PAS）与macconill - Solcia的铅苏木精结合，在我们的研究中被证明是可靠的，可用于绘制这些细胞沿气道分布的连续2‐m´m甲基丙烯酸乙二醇切片的常规染色。在小鼠、仓鼠、小猫和胎兔的肺中，典型的小颗粒细胞仅用苏木精铅染色很弱或根本不染色，因此很容易被忽视。PAS使细胞质呈弥漫性品红色；由于它具有淀粉酶抗性，不如黏液那么亮，但比细支气管细胞分泌物更亮，并且比其他细胞的溶酶体染色更精细，因此其效果是突出小颗粒细胞，无论是孤立的还是聚集的。基底膜的PAS染色和纤毛的苏木精铅染色增强了联合染色的分辨能力。在甲状腺中，滤泡旁细胞与滤泡细胞相对抗；在小肠中，苏木精阳性内分泌细胞与周围的吸收细胞、黏液细胞和潘氏细胞分化良好。利用互补单胺荧光技术对对照和5 -羟色氨酸预处理动物的肺塑料切片进行染色，我们可以发现荧光上皮细胞与PAS -铅hema - toxylin染色的细胞是相同的。
Epithelial cells of the several subtypes that comprise the small‐granule cell population of the respiratory system are little studied, partly because adequate silver, monoamine fluorescence and other specific light microscopical preparations have been more difficult to obtain than in the gut and other organs possessing diffuse endocrine systems. Periodic acid‐Schiff (PAS) in combination with MacConaill‐Solcia's lead hematoxylin has in our hands proven dependable for routine staining of serial 2‐m̈m glycol methacrylate sections used in mapping the distributions of these cells along the airway. In lungs of mice, hamsters, kittens, and fetal rabbits, typical small‐granule cells stain weakly or not at all with lead hematoxylin alone, hence are easily overlooked. PAS adds to the cytoplasm a diffuse magenta coloration; and because it is diastase‐resistant, less brilliant than that of mucus but more so than bronchiolar cell secretions, and finer textured than lysosomal staining of other cells present, the effect is to highlight small‐granule cells whether solitary or in clusters. Additional PAS staining of basement membranes and lead hematoxylin staining of cilia enhance the combined stain's resolving power. In thyroid gland, parafollicular cells stand out boldly against follicular elements; in small intestine, hematoxylin‐positive endocrine cells are well differentiated from absorptive, mucous, and Paneth cells that surround them. Using a complementary monoamine fluorescence technique on plastic sections of lungs from control and 5‐hydroxytryptophan‐pretreated animals prior to staining, we can show that fluorescent epithelial cells are identical with those stained by PAS‐lead hema‐toxylin.