SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis.
SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis.
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SMYD1 是 SRF 的相互作用伙伴，参与血管生成
DOI：
10.1371/journal.pone.0146468
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Ye X;Qian Y;Wang Q;Yuan W;Mo X;Li Y;Jiang Z;Xu W;Deng Y;Wan Y;Fan X;Wu X;Wang Y
已有研究表明Smyd 1在心肌细胞分化、心肌形态发生和肌原纤维组织中起着重要作用。在这项研究中，我们发现了一个新的功能Smyd 1的调节内皮细胞（ECs）。我们的数据显示Smyd 1在血管内皮细胞中表达，并且在内皮细胞中敲低SMYD 1会损害EC迁移和管形成。此外，Co-IP和GST pull-down分析表明SMYD 1与血清反应因子（SRF）相关。EMSA分析进一步表明SMYD 1与SRF形成复合物并增强SRF DNA结合活性。我们的研究表明，SMYD 1作为SRF相互作用蛋白，增强SRF DNA结合活性，并需要EC迁移和管形成，以调节血管生成。
Previous studies have demonstrated that Smyd1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization. In this study, we uncovered a novel function of Smyd1 in the regulation of endothelial cells (ECs). Our data showed that Smyd1 is expressed in vascular endothelial cells, and knockdown of SMYD1 in endothelial cells impairs EC migration and tube formation. Furthermore, Co-IP and GST pull-down assays demonstrated that SMYD1 is associated with the Serum Response Factor (SRF). EMSA assays further showed that SMYD1 forms a complex with SRF and enhances SRF DNA binding activity. Our studies indicate that SMYD1 serves as an SRF-interacting protein, enhances SRF DNA binding activity, and is required for EC migration and tube formation to regulate angiogenesis.