Molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under neutral pH
Molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under neutral pH
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具有多硼酸位点的分子印迹胶体阵列用于中性pH下糖蛋白检测
DOI：
10.1016/j.jcis.2021.09.048
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发表时间：
2021-09-24
影响因子：
9.9
通讯作者：
Fu, Min
中科院分区：
文献类型：
--
作者：
Chen, Wei;Guo, Zhiyang;Fu, Min
糖蛋白在生物体中起着至关重要的作用，并且经常作为某些疾病的生物标志物。然而，由于生物体液中糖蛋白的含量低，糖蛋白的选择性检测仍然是一个具有挑战性的问题，需要解决。本研究提出了一种在生理pH条件下检测糖蛋白的多硼酸位点分子印迹胶体阵列。以辣根过氧化物酶（HRP）为模板，聚乙烯亚胺（PEI）增加硼酸基团的数目，采用表面印迹法制备了单分散的糖蛋白印迹粒子（SiO2@PEI/MIPs）。结合实验表明，SiO2@PEI/MIPs对HRP具有良好的吸附容量（1.41 lmol/g）、快速的吸附速率（40 min）和较好的选择性。然后将SiO2@PEI/MIPs组装成紧密堆积的胶体阵列，构建了无标记的光学传感器（GICA）。由于GICA具有高度有序的光子晶体结构，HRP与GICA的结合可以通过结构颜色和衍射波长的变化直接读出。当HRP浓度从2.5增加到15 lmol/L时，GICA的结构颜色由亮蓝色变为黄色，衍射波长红移59 nm。重要的是，GICA能够从人血清样品中检测HRP。所有这些结果表明，GICA的糖蛋白的肉眼检测的潜力。(C)2021爱思唯尔公司All rights reserved.
Glycoproteins play vital roles in living organisms and often serve as biomarkers for some disease. However, due to the low content of glycoprotein in biological fluids, selective detection of glycoproteins is still a challenging issue that needs to be addressed. In this study, molecularly imprinted colloidal array with multi-boronic acid sites for glycoprotein detection under physiological pH was proposed. Monodispersed glycoprotein imprinted particles (SiO2@PEI/MIPs) was first prepared based on surface imprinting strategy using horseradish peroxidase (HRP) as template, and polyethyleneimine (PEI) was used to increase the number of boronic acid groups. The binding experiment indicated that the SiO2@PEI/MIPs hold satisfactory adsorption capacity (1.41 lmol/g), rapid adsorption rate (40 min) and preferable selectivity toward HRP. Then the SiO2@PEI/MIPs was assembled into close-packed colloidal array to construct a label free optical sensor (denoted as GICA). Benefiting from the high ordered photonic crystal structure, binding of HRP onto the GICA could be directly readout from the changes in structure color and diffracted wavelength. The structure color of the GICA changed from bright blue to yellow with the diffraction wavelength red shifted 59 nm when the HRP concentration increased from 2.5 to 15 lmol/L. Importantly, the GICA was capable of detecting HRP from human serum samples. All those results indicated the potential of the GICA for naked-eye detection of glycoprotein. (C) 2021 Elsevier Inc. All rights reserved.