UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.
UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA.
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DOI：
10.1016/j.cub.2009.09.041
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发表时间：
2009-12-01
期刊：
影响因子：
--
通讯作者：
Wilson SA
中科院分区：
文献类型：
--
作者：
Hautbergue GM;Hung ML;Walsh MJ;Snijders AP;Chang CT;Jones R;Ponting CP;Dickman MJ;Wilson SA
信使RNA(Messenger RNA，mRNA)输出接头在mRNA从细胞核到细胞质的运输过程中发挥着重要作用。它们将早期的mRNA加工事件，如5‘端封顶和3’端形成与TAP/Nxf1输出受体加载到mRNA上。正则适配子REF/Aly/Yra1通过UAP56被招募到信使核糖核酸，随后将信使核糖核酸传递给Nxf1。在高等真核生物中，UAP56和Nxf1的敲除有效地阻止了mRNA的输出，而REF的敲除仅导致轻微的减少，这表明存在额外的接头。在这里，我们发现了一个新的UAP56相互作用因子，UIF，它作为一个出口适配器，结合Nxf1并将mRNA运送到核孔中。REF和UIF同时存在于同一个信使核糖核酸分子上，这两种蛋白质都是有效输出信使核糖核酸所必需的。我们发现组蛋白伴侣事实通过SSRP1亚基与UIF特异性结合，但不与REF结合，这种相互作用是UIF募集到mRNA所必需的。综上所述，结果表明REF和UIF是通过UAP56-Nxf1途径输出细胞mRNAs的关键人类适配器。
Messenger RNA (mRNA) export adaptors play an important role in the transport of mRNA from the nucleus to the cytoplasm. They couple early mRNA processing events such as 5′ capping and 3′ end formation with loading of the TAP/NXF1 export receptor onto mRNA. The canonical adaptor REF/ALY/Yra1 is recruited to mRNA via UAP56 and subsequently delivers the mRNA to NXF1. Knockdown of UAP56 and NXF1 in higher eukaryotes efficiently blocks mRNA export, whereas knockdown of REF only causes a modest reduction, suggesting the existence of additional adaptors. Here we identify a new UAP56-interacting factor, UIF, which functions as an export adaptor, binding NXF1 and delivering mRNA to the nuclear pore. REF and UIF are simultaneously found on the same mRNA molecules, and both proteins are required for efficient export of mRNA. We show that the histone chaperone FACT specifically binds UIF, but not REF, via the SSRP1 subunit, and this interaction is required for recruitment of UIF to mRNA. Together the results indicate that REF and UIF represent key human adaptors for the export of cellular mRNAs via the UAP56-NXF1 pathway.
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