Recombinant allergens promote expression of CD203c on basophils in sensitized individuals
Recombinant allergens promote expression of CD203c on basophils in sensitized individuals
复制标题
DOI：
10.1067/mai.2002.125257
复制
发表时间：
2002-07-01
影响因子：
14.2
通讯作者：
Valent, P
中科院分区：
文献类型：
--
作者：
Hauswirth, AW;Natter, S;Valent, P
背景：传统上，I型过敏症的诊断是基于临床数据、皮肤测试结果和过敏原提取物的实验室测试结果。在过去的几年里，人们试图通过引入重组变应原和新的IgE依赖细胞激活的标志物来完善临床变态反应的诊断方法。目的：我们已经鉴定了胞外酶CD203c是一种新的嗜碱性细胞抗原，它上调在IgE受体的交链上。在这项研究中，应用CD203c和一组重组变应原建立了一种新的嗜碱性细胞试验，允许在效应细胞水平上可靠地定量IgE依赖的反应。方法：对白桦树(Bet v，1，n=15；Bet v 2，n=8)和草(Phl p1，n=15；Phl p2，n=10；Phl p5，n=14)过敏的患者以及10名非过敏者进行了花粉变应原检测。将嗜碱性粒细胞暴露于不同浓度的重组变应原15min后，用流式细胞仪检测CD203c的表达。结果：重组变应原可使致敏者外周血嗜碱性粒细胞CD203c表达呈剂量依赖性增加，而健康对照组未见明显增加。重组变应原对CD203c表达的影响也具有时间依赖性。变应原诱导的CD203c表达上调与CD63表达上调有很好的相关性(R=0.76)。结论：重组变应原诱导的嗜碱性粒细胞表面CD203c表达的流式细胞术定量检测是一种有效的检测致敏个体过敏状态的方法，为敏感的新型变态反应试验奠定了基础。
Background: Traditionally, the diagnosis of type I allergies is based on clinical data, skin test results, and laboratory test results with allergen extracts. During the past few years, several attempts have been made to refine diagnostic assays in clinical allergy by introducing recombinant allergens and novel markers of IgE-dependent cell activation.Objectives: We have identified the ectoenzyme CD203c as a novel basophil antigen that is upregulated on IgE receptor cross-linkage. In this study A e applied CD203c and a panel of recombinant allergens to establish a novel basophil test that,allows for a reliable quantification of IgE-dependent responses at the effector cell level.Methods: Patients allergic to birch (Bet v, 1, n = 15; Bet v 2, n := 8) and grass (Phl p 1, n = 15; Phl p 2, n = 10; Phl p 5, n = 14) pollen allergens, as well as 10 nonallergic donors, were examined. Basophils were exposed to various concentrations of recombinant allergens for 15 minutes and then examined for expression of CD203c by means of flow cytometry. CD203c upregulation was correlated with the increase in CD63.Results: Exposure to recombinant allergens resulted in a dose-dependent increase in expression of CD203c on peripheral blood basophils in sensitized individuals, whereas no increase,was seen in healthy control subjects. The effects of the recombinant allergens on CD203c expression were also time dependent. There was a good correlation between allergen-induced upregulation of CD203c and upregulation of CD63 (R = 0.76).Conclusion: Flow cytometric quantitation of CD203c on blood basophils exposed to recombinant allergens is a useful approach to determine the allergic state in sensitized individuals and represents a basis for a sensitive novel allergy test.