Molecular Characterization of the Genital Organizer: Gene Expression Profile of the Mouse Urethral Plate Epithelium.
Molecular Characterization of the Genital Organizer: Gene Expression Profile of the Mouse Urethral Plate Epithelium.
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生殖器组织者的分子特征：小鼠尿道板上皮的基因表达谱。
DOI：
10.1016/j.juro.2016.04.091
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Cohn,MartinJ
中科院分区：
文献类型：
--
作者：
Armfield,BrookeA;Seifert,AshleyW;Zheng,Zhengui;Merton,EmilyM;Rock,JasonR;Lopez,Maria-Cecilia;Baker,HenryV;Cohn,MartinJ
Purpose:Lower urinary tract malformations are among the most common congenital anomalies in humans. Molecular genetic studies of mouse external genital development have begun to identify mechanisms that pattern the genital tubercle and orchestrate urethral tubulogenesis. The urethral plate epithelium is an endodermal signaling region that has an essential role in external genital development. However, little is known about the molecular identity of this cell population or the genes that regulate its activity.Materials and Methods:We used microarray analysis to characterize differences in gene expression between urethral plate epithelium and surrounding tissue in mouse genital tubercles. In situ hybridizations were performed to map gene expression patterns and ToppCluster (https://toppcluster. cchmc. org/) was used to analyze gene associations.Results:A total of 84 genes were enriched at least 20-fold in urethral plate epithelium relative to surrounding tissue. The majority of these genes were expressed throughout the urethral plate in males and females at embryonic day 12.5 when the urethral plate is known to signal. Functional analysis using ToppCluster revealed genetic pathways with known functions in other organ systems but unknown roles in external genital development. Additionally, a 3-dimensional molecular atlas of genes enriched in urethral plate epithelium was generated and deposited at the GUDMAP (GenitoUrinary Development Molecular Anatomy Project) website (http://gudmap. org/).Conclusions:We identified dozens of genes previously unknown to be expressed in urethral plate epithelium at a crucial developmental period. It provides a novel panel of genes for analysis in animal models and in humans with external genital anomalies.
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影响因子：
4
作者：
Kaestner, Klaus H.
通讯作者：
Kaestner, Klaus H.
影响因子：
2.7
作者：
Chiu HS;Szucsik JC;Georgas KM;Jones JL;Rumballe BA;Tang D;Grimmond SM;Lewis AG;Aronow BJ;Lessard JL;Little MH
通讯作者：
Little MH
大鼠阴茎骨骼组织发育的近远端顺序及上皮的诱导作用。
DOI：
--
发表时间：
1986
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
R. Murakami;T. Mizuno
通讯作者：
T. Mizuno
影响因子：
4.6
作者：
Miyagawa, Shinichi;Moon, Anne;Yamada, Gen
通讯作者：
Yamada, Gen
影响因子：
2.7
作者：
Ching, Saunders T.;Cunha, Gerald R.;Baskin, Laurence S.;Basson, M. Albert;Klein, Ophir D.
通讯作者：
Klein, Ophir D.