A simple disc degeneration model induced by percutaneous needle puncture in the rat tail
A simple disc degeneration model induced by percutaneous needle puncture in the rat tail
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DOI：
10.1097/brs.0b013e31817c64a9
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发表时间：
2008-08-15
期刊：
影响因子：
3
通讯作者：
Chen, Qi-xin
中科院分区：
文献类型：
--
作者：
Han, Bin;Zhu, Kai;Chen, Qi-xin
研究设计.目的观察经皮穿刺大鼠尾椎椎间盘的退变情况，比较2种穿刺方式的穿刺深度和椎间盘退变率。建立一种简单的椎间盘退变动物模型。退行性椎间盘疾病的生物学治疗研究主要依赖于动物模型。在兔腰椎中的环形针刺诱导椎间盘退变已被证明是成功的，但类似的方法尚未在大鼠尾盘中进行评估，即使它可能产生一个理想的模型用于椎间盘修复研究。两个连续的大鼠尾椎间盘，近端和远端的第八尾椎，随机损伤和对照。用20号针以完全穿透或半穿透的方式穿刺选择用于损伤的椎间盘。在1、2和4周后收获椎间盘。测量包括钼靶数字X线片上的椎间盘高度、生物化学（水含量、糖胺聚糖和羟脯氨酸）和组织学。全针或半针穿刺分别在伤后1周和2周引起明显的椎间隙狭窄和进行性的组织学退变。半穿透穿刺椎间盘在4周时，穿透穿刺椎间盘在2周和4周时观察到糖胺聚糖含量显著降低。穿透穿刺导致椎间盘高度下降更快，糖胺聚糖含量更低，组织学变性程度更高。椎间盘的含水量和羟脯氨酸含量无明显变化。尾椎间盘穿刺是一种简单易行的椎间盘退变方法，退变率与穿刺深度呈正相关。该模型仍有一些局限性，在解释和外推该模型中的椎间盘再生研究结果时应考虑到这一点。
Study Design. We evaluated the degenerative changes to rat tail vertebral discs induced by percutaneous needle puncture, and we compared 2 puncture styles for the depth of needle puncture and the rate of disc degeneration.Objective. To develop a simple animal model of disc degeneration.Summary of Background Data. The study of biologically based treatments for degenerative disc disease depends largely on animal models. Annulus needle puncture in the lumbar spine inducing disc degeneration in rabbits has proven successful, but a similar method has not been evaluated in the tail discs of rats, even though it might produce a desirable model for disc repair studies.Methods. Two consecutive rat tail vertebral discs, proximal and distal to the eighth coccygeal vertebra, were randomized for injury and control. The disc selected for injury was punctured percutaneously using a 20- gauge needle with either full penetration or half penetration. The discs were harvested 1, 2, and 4 weeks later. Measurements included disc height on molybdenum target digital radiographs, biochemistry (water content, glycosaminoglycans, and hydroxyproline), and histology.Results. Needle punctures with full or half penetration caused significant disc space narrowing and progressive histologic changes of degeneration as early as 1 and 2 weeks after injury, respectively. Significant decrease in glycosaminoglycan content was observed at 4 weeks in the half- penetration puncture discs and at 2 and 4 weeks in discs punctured penetratively. Penetrative puncture resulted in a faster decrease in disc height, lower glycosaminoglycan content, and higher grades of histologic degeneration. The water and hydroxyproline content of the discs did not change appreciably.Conclusion. Tail disc percutaneous needle puncture is a simple method for inducing disc degeneration and the rate of degeneration is positively related to the depth of needle puncture. This model still has some limitations that should be taken into consideration when results of disc regeneration research in this model are interpreted and extrapolated to human.