Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate
Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate
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DOI：
10.1038/nature07017
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发表时间：
2008-06-12
期刊：
影响因子：
64.8
通讯作者：
Brindle, Kevin M.
中科院分区：
文献类型：
--
作者：
Gallagher, Ferdia A.;Kettunen, Mikko I.;Brindle, Kevin M.
由于组织pH值的变化是许多病理过程的基础，因此在临床中对组织pH值进行成像的能力可以提供检测疾病和治疗反应的新方法（1）。动态核极化是一种新兴技术，可大幅提高磁共振成像实验的灵敏度（2，3）。在这里，我们表明，组织的pH值可以在体内成像的超极化碳酸氢盐（（HCO 3-）-C-13）和（CO2）-C-13的信号强度的比率静脉注射超极化（HCO 3-）-C-13。该技术在小鼠肿瘤模型中得到了证实，该模型显示肿瘤间质的平均pH值显著低于周围组织。考虑到碳酸氢盐是一种内源性分子，可以以相对高的浓度输注到患者体内（4），我们建议该技术可以在临床上用于成像与组织pH值变化相关的病理过程，如癌症，缺血和炎症。
As alterations in tissue pH underlie many pathological processes, the capability to image tissue pH in the clinic could offer new ways of detecting disease and response to treatment(1). Dynamic nuclear polarization is an emerging technique for substantially increasing the sensitivity of magnetic resonance imaging experiments(2,3). Here we show that tissue pH can be imaged in vivo from the ratio of the signal intensities of hyperpolarized bicarbonate ((HCO3-)-C-13) and (CO2)-C-13 following intravenous injection of hyperpolarized (HCO3-)-C-13. The technique was demonstrated in a mouse tumour model, which showed that the average tumour interstitial pH was significantly lower than the surrounding tissue. Given that bicarbonate is an endogenous molecule that can be infused in relatively high concentrations into patients(4), we propose that this technique could be used clinically to image pathological processes that are associated with alterations in tissue pH, such as cancer, ischaemia and inflammation.