Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton.
Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton.
复制标题
与棉花抗黄萎病和非生物胁迫相关的新型 NAC 基因的表征、表达和功能分析
DOI：
10.1534/g3.116.034512
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发表时间：
2016-12-07
期刊：
影响因子：
--
通讯作者：
Cai Y
中科院分区：
文献类型：
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作者：
Wang W;Yuan Y;Yang C;Geng S;Sun Q;Long L;Cai C;Chu Z;Liu X;Wang G;Du X;Miao C;Zhang X;Cai Y
阐明棉花对生物和非生物胁迫的抗性机制具有重要意义。本研究从海岛棉（Gossypium barbadense L.）同源序列比对表明GbNAC1属于TERN亚群。GbNAC1蛋白定位于细胞核。GbNAC1在根、茎和叶中表达，并且在维管束中表达尤其高。功能分析表明，利用病毒诱导的基因沉默（VIGS）方法沉默GbNAC1基因后，棉花对黄萎病的抗性降低。与野生型相比，GbNAC1过表达的拟南芥表现出对大丽轮枝菌的抗性增强。因此，GbNAC1参与了水稻抗黄萎病的正向调控。此外，在不同胁迫处理下对GbNAC1过表达拟南芥的分析表明，它参与植物生长、发育和对各种非生物胁迫（阿坝、甘露醇和NaCl）的响应。这表明GbNAC 1在棉花抗生物和非生物胁迫中起重要作用。本研究为进一步研究NAC基因在棉花等植物中的功能奠定了基础。
Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.