Ultrasonic Microelastography to Assess Biomechanical Properties of the Cornea.
Ultrasonic Microelastography to Assess Biomechanical Properties of the Cornea.
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DOI：
10.1109/tbme.2018.2853571
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Zhou Q
中科院分区：
文献类型：
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作者：
Qian X;Ma T;Shih CC;Heur M;Zhang J;Shung KK;Varma R;Humayun MS;Zhou Q
通过超声微弹性成像系统对角膜生物力学特性进行定性和定量研究，在圆锥角膜、屈光后角膜扩张等疾病的诊断和交联手术等跟踪治疗中具有潜在的应用价值。我们的成像系统具有双频配置，包括用于推动组织的4.5MHz环形换能器和用于跟踪微米级位移的共焦对准的40MHz针换能器。在离体猪角膜上进行2D/3D声辐射力脉冲（ARFI）成像和感兴趣区域的杨氏模量，所述离体猪角膜使用福尔马林溶液交联或预加载有5 mmHg至30 mmHg的IOP。在ARFI图像中可以精确地观察到福尔马林交联后角膜硬度的增加和交联体积的变化，并通过重建的杨氏模量反映出来，而B型结构图像几乎没有变化。此外，在12只猪角膜中研究了角膜硬度与IOP之间的关系。角膜硬度在不同IOP下显著不同，并且具有随着IOP增加而变得更硬的趋势。我们的研究结果表明，使用超声微弹性成像技术的角膜的生物力学特性的图像的原则。将标记有重建杨氏模量的高分辨率ARFI成像与角膜的结构成像相结合可能会导致眼科领域中常规执行的成像模式。
to both qualitatively and quantitatively investigate corneal biomechanical properties through an ultrasonic micro-elastography imaging system, which is potentially useful in diagnosis of diseases such as keratoconus, post-refractive keratectasia and tracking treatment such as cross-linking surgery. our imaging system has a dual frequency configuration, including a 4.5 MHz ring transducer to push the tissue and a confocally aligned 40 MHz needle transducer to track micron-level displacement. 2D/3D acoustic radiation force impulse (ARFI) imaging and the Young’s modulus in the region of interest were performed on ex vivo porcine corneas that were either cross-linked using formalin solution or preloaded with IOPs from 5 mmHg to 30 mmHg. the increase of corneal stiffness and the change in cross-linked volume following formalin crosslinking could be precisely observed in the ARFI images and reflected by the reconstructed Young’s modulus while the B-mode structural images remained almost unchanged. In addition, the relationship between the stiffness of the cornea and IOPs was investigated among twelve porcine corneas. The corneal stiffness is significantly different at various IOPs and has a tendency to become stiffer with increasing IOP. Our results demonstrate the principle of using ultrasonic micro-elastography techniques to image the biomechanical properties of the cornea. Integrating high-resolution ARFI imaging labelled with reconstructed Young’s modulus and structural imaging of the cornea can potentially lead to a routinely performed imaging modality in the field of ophthalmology.