Stevioside inhibits inflammation and apoptosis by regulating TLR2 and TLR2-related proteins in S. aureus-infected mouse mammary epithelial cells.
Stevioside inhibits inflammation and apoptosis by regulating TLR2 and TLR2-related proteins in S. aureus-infected mouse mammary epithelial cells.
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DOI：
10.1016/j.intimp.2014.06.015
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发表时间：
2014-09
影响因子：
5.6
通讯作者：
Tiancheng Wang;Xiaojin Song;Ze-cai Zhang;Meng-yao Guo;Haichao Jiang;Wei Wang;Yongguo Cao;Lianqin Zhu;Naisheng Zhang
中科院分区：
文献类型：
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作者：
Tiancheng Wang;Xiaojin Song;Ze-cai Zhang;Meng-yao Guo;Haichao Jiang;Wei Wang;Yongguo Cao;Lianqin Zhu;Naisheng Zhang
甜菊糖苷是一种天然甜味剂，通常用于传统医药和食品添加剂。本研究旨在探讨甜菊糖苷对金黄色葡萄球菌的抗炎和抗凋亡作用及其可能的分子机制。金黄色葡萄球菌感染小鼠乳腺上皮细胞（MMECs）。在感染s之前，用不同剂量的甜菊糖苷处理细胞。葡萄球菌。免疫荧光显微镜检测活/死细胞。ELISA法检测促炎因子。采用q-PCR分析TLR2 mRNA及NF-κB、MAPK、凋亡相关蛋白的表达。Western blot检测相对蛋白表达水平。结果表明，甜菊糖甙抑制TNF-α、IL-6和IL-1β剂量依赖性inS的mRNA和蛋白表达。aureus-stimulated MMECs。甜菊苷抑制了这些。TLR2和NF-κB、MAPK通路蛋白的表达以及凋亡。IκBα、p38、ERK、JNK、p65、caspase-3和Bax mRNA水平不受甜菊苷处理的影响。甜菊苷通过抑制细胞因子的释放和TLR2、NF-κB和MAPK信号通路蛋白以及caspase-3和Bax的激活，发挥抗炎和抗凋亡的作用。
Stevioside is a natural sweetener that is commonly used in traditional medicine and as a food additive. The object of this study was to investigate the anti-inflammatory and anti-apoptosis function of stevioside and the possible molecular mechanisms for such activity in Staphylococcus aureus(S. aureus)-infected mouse mammary epithelial cells (MMECs). The cells were treated with varying doses of stevioside before infection withS. aureus. The live/dead cells were detected by immunofluorescence microscopy. The pro-inflammatory cytokines were determined by ELISA. The mRNA of TLR2 and proteins related to NF-κB, MAPK and apoptosis were analyzed by q-PCR. The relative protein expression levels were determined by Western blot. The results indicated that stevioside inhibited the mRNA and protein expression of TNF-α, IL-6 and IL-1β dose-dependently inS. aureus-stimulated MMECs. Stevioside suppressed theS. aureus-induced expression of TLR2 and proteins of the NF-κB and MAPK pathways as well as apoptosis. The mRNA levels of IκBα, p38, ERK, JNK, p65, caspase-3 and Bax were not influenced by the stevioside treatment. Stevioside exerts anti-inflammatory and anti-apoptotic properties by inhibiting the release of cytokines and the activation of TLR2 and proteins of the NF-κB and MAPK signaling pathways, as well as caspase-3 and Bax.